Non-Invasive Oxygen Sensors
Robust & real conditions: Look into any transparent vessel
- Glassware & disposables
- Bags & single-use bioreactors
- PET & glass bottles
The non-invasive optical oxygen sensors measure the partial pressure of both dissolved and gaseous oxygen. These sensor spots are used for glassware and disposables. The sensor spots are fixed on the inner surface of the glass or transparent plastic material. The oxygen concentration can therefore be measured in a non-invasive and non-destructive manner from outside, through the wall of the vessel. Different coatings for different concentration ranges are available.
Features
- Online monitoring
- Non-invasive & non-destructive measurement
- Measurement range from 1ppb up to 45 ppm dissolved oxygen
- No consumption of oxygen
- Signal independent on flow velocity
- Measures oxygen in liquids as well as in gas phase
- Autoclavable (SIP: 130 °C / 266°F, 2 atm steam sterilization) & CIP (Cleaning in place)
Selected Probes
Sensor Spots
Sensor spots (SP) are the most versatile version of non-invasive oxygen sensors. They are attached to the inner surface of any transparent vessel.
Examples are
- Cultivation bags
- Spinners
- Glass reactors
The transmitter with its optical fiber can be fixed opposite the sensor spot by using our accessories (see accessories brochure) which can be adapted for nearly all kinds of vessels.
Flow-Through Cell
The flow-through oxygen minisensor (FTC) is a miniaturized fiber chemical optical sensor integrated in a flow-through cell. It is connected to the transmitter by an optical fiber. A glass tube with an inner diameter of 2 mm is coated with an oxygen sensor at its inner wall. The volume for liquid inside the FTC is about 100 (±10) microliter. The standard flow cell can be easily connected via Luer-Lock adapters to external tubings.
The Smart Measurement Method
The light from the blue LED excites the sensor spot to emit fluorescence. If the sensor spot encounters an oxygen molecule, the excess energy is transferred in a non-radiative way, decreasing or quenching the fluorescence signal. The degree of quenching correlates to the partial pressure of oxygen in the matrix, which is in dynamic equilibrium with oxygen in the sample. The decay time measurement is internally referenced.
Examples for Applications
Pharma Industry: Oxygen Monitoring in Bags
Bags and single-use bioreactors are in the process of revolutionising the way biopharmaceuticals are manufactured. Our non-invasive oxygen sensors are the tools to make the cultivation vessels fully disposable. As non-invasive pH sensors are also available, the two key parameters oxygen and pH can be controlled online.
Food & Beverage: Oxygen Permeation Measurement in PET Bottles
Non-invasive oxygen sensors measure both in liquid and in gaseous (headspace) phases. They perform through transparent materials up to a thickness of 10 mm and even through slightly opaque packaging. The measurement is carried out by firmly holding the fiber optic probe against the side of a PET bottle where the sensor spot is positioned. Adjustable mountings and bespoke fixtures are available. This system allows even the parallel measurement of different bottles as the fiber can be moved from bottle to bottle.
Bioprocess Development: Oxygen Monitoring in Shake Flasks
O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.
Transmitters & Accessories
Fibox 3/
Fibox 3 trace
Fiber optic oxygen transmitter
OXY-4 mini/
OXY-4 trace
4-channel fiber optic oxygen transmitter
OXY-10 mini/
OXY-10 trace
10-channel fiber optic oxygen transmitter
Fibox 3 LCD/
Fibox 3 LCD trace
Fiber optic oxygen transmitter with LCD display
LP-1 Control Panel
The control panel is a device for controlling single-channel oxygen transmitters.
Respirometer Chambers
O2 consumption measurements in aquatic organisms and solutions.
Automated Respirometry
Automated measurements of O2 consumption rates in aquatic organisms.
Accessories
A variety of accessories like connectors to different vessels is available.
