Non-Invasive Optical Oxygen Sensors
Robust & real conditions: Look into any transparent vessel
- Glassware & disposables
- Bags & single-use bioreactors
- PET & glass bottles
The non-invasive optical oxygen sensors measure the partial pressure of both dissolved and gaseous oxygen. These sensor spots are used for glassware and disposables. The optical oxygen sensors are fixed on the inner surface of the glass or transparent plastic material. The oxygen concentration can therefore be measured in a non-invasive and non-destructive manner from outside, through the wall of the vessel. Different coatings for different concentration ranges are available.
- Non-invasive & non-destructive online measurement
- Measurement range from 1 ppb up to 45 ppm dissolved oxygen
- Ultra low gaseous oxygen measurement down to 0.5 ppm
- No consumption of oxygen
- Signal independent of flow velocity
- Measures oxygen in liquids as well as in gas phase
- Autoclavable (SIP: 130 °C / 266°F, 2 atm steam sterilization) & CIP (Cleaning in place)
Sensor Spots O2
Sensor spots (SP) are the most versatile version of non-invasive optical oxygen sensors. They are attached to the inner surface of any transparent vessel.
- Cultivation bags
- Glass reactors
The transmitter with its optical fiber can be fixed opposite the sensor spot by using our accessories (see accessories brochure) which can be adapted for nearly all kinds of vessels.
Flow-Through Cell O2
The flow-through oxygen minisensor (FTC) is a miniaturized chemical optical sensor integrated in a flow-through cell. It is connected to the transmitter by an optical fiber. A glass tube with an inner diameter of 2 mm is coated with an optical oxygen sensor at its inner wall. The volume for liquid inside the FTC is about 100 (±10) microliter. The standard flow-through cell can be easily connected via Luer-Lock adapters to external tubings.
SensorVial & Vial Adapter
The SensorVial is a 20 mL glass vial with an integrated optical oxygen sensor stripe. It is connected to the transmitter by an optical fiber. The Vial Adapter holds the fiber in place and is adjustable in different heights. 2 different versions of the SensorVial are available: For stirred application the sensor stripe does not reach all the way to the vial bottom, while for non-stirred application the sensor stripe covers the whole vial side.
Examples for Applications
Pharma Industry: Oxygen Monitoring in Bags
Bags and single-use bioreactors are in the process of revolutionizing the way biopharmaceuticals are manufactured. Our non-invasive oxygen sensors are the tools to make the cultivation vessels fully disposable. As non-invasive pH sensors are also available, the two key parameters oxygen and pH can be controlled online.
Food & Beverage: Oxygen Permeation Measurement in PET Bottles
Non-invasive oxygen sensors measure both in liquid and in gaseous (headspace) phases. They perform through transparent materials up to a thickness of 10 mm and even through slightly opaque packaging. The measurement is carried out by firmly holding the fiber optic probe against the side of a PET bottle where the sensor spot is positioned. Adjustable mountings and bespoke fixtures are available. This system allows even the parallel measurement of different bottles as the fiber can be moved from bottle to bottle.
Bioprocess Development: Oxygen Monitoring in Shake Flasks
O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.
Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials
Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.
Please click to enlarge.
Transmitters & Accessories
Stand-alone fiber optic oxygen transmitter
Stand-alone fiber optic oxygen transmitter
4-channel fiber optic oxygen transmitter
10-channel fiber optic oxygen transmitter
Fiber optic oxygen transmitter with LCD display
The control panel is a device for controlling single-channel oxygen transmitters.
O2 consumption measurements in aquatic organisms and solutions.
Automated measurements of O2 consumption rates in aquatic organisms.
A variety of accessories like connectors to different vessels is available.