Selected Probes

Software

The SFR Software SFRS is the integrated control center for the SFR Shake Flask Reader. In addition, it offers an integrated database to show and evaluate measurements. The connection between PC and SFR is wireless. Oxygen and pH are visualized in real-time during the entire cultivation. Results are displayed in a variety of graphical representations. All measured data can be exported to Excel® or as .csv for further evaluation. In addition, the current measurement can be compared to stored cultivations online.

Benefits

• Enables process monitoring in shake flasks
• Systematic optimization of cultivation parameters
• Reliable comparison of screening results
• Enhanced culture quality and efficiency
• Real-time data acquisition
• Compliant with FDA 21 CRF part 11
• Compatible with established protocols
• Supports finding feeding points
• Facilitates optimization of harvesting strategies

The Smart Measurement Method

The sensor spots are fixed at the bottom of the shake flasks. The luminescence of the dyes embedded in these sensor spots is excited by the Shake Flask Reader. The luminescence lifetime is detected non-invasively through the transparent flask. The luminescence lifetime of the sensor dye depends on the oxygen partial pressure and the pH of the sample, respectively. It is converted to oxygen and pH values by the software using the provided calibration data and internally stored conversion formula.