Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
Find Publications and Information about Your Optical Sensor System
Whether it is general information or detailed, application based data on optical sensors you are looking for, in our collection of publications, sensor basics and FAQs you will find what you need. Furthermore, short tutorial videos and our instruction manuals will help you with handling your sensor system.
O2-sensitive Microcavity Arrays for 3D Cell Culture Monitoring: Oxygen Imaging in Spheroids with VisiSens TD Technology
C. Grün1, G. Liebsch2 und E. Gottwald1
1Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Microthermoforming is applied to create an array of microcavities in oxygen sensor foils. 3D cell aggregates can be cultured in these cavities and the oxygen content can be…
Download PDFOnline Monitoring of C. glutamicum Culture in Ultra Yield® Flasks: SFR vario Used with Only Slightly Translucent Shake Flasks
C. Ude and G. T. John
PreSens Precision Sensing GmbH, Regensburg, Germany
In this study we analyzed, if slightly translucent polypropylene shake flasks can be used for optical measurements and especially optical biomass monitoring with the SFR vario. As…
Download PDFWhy It Is Important to Measure Different Parameters Online in Shake Flasks: SFR vario Measures O2, OUR, pH and Biomass Online
R. W. Maschke1, G. T. John2, and D. Eibl1
1 ZHAW Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Wädenswil, Switzerland
2PreSens Precision Sensing GmbH, Regensburg, Germany
Optical sensors enable monitoring and controlling important culture parameters even at shake flask scale. In this short report we show an example of multiparameter monitoring with…
Download PDFO2, pH and CO2 Monitoring in Microfluidic Mammalian Cell Culture: Cultivated Meat Bioprocess Optimization with SensorPlugs
Vasa Radonic, Ivana Podunavac, Mila Djisalov, Teodora Knezic, Ivana Gadjanski
BioSense Institute, Novi Sad, Serbia
We tested the efficacy of PreSens SensorPlugs for monitoring mammalian cell culturing processes in microfluidic bioreactors (MBs) with integrated sensors. MRC-5 fibroblasts (ATCC®…
Download PDFCultivated Meat Bioprocess Optimization: O2, pH and CO2 Monitoring in E. coli Culture with PreSens SensorPlugs
Vasa Radonic, Ivana Podunavac, Mila Djisalov, Teodora Knezic, Ivana Gadjanski
BioSense Institute, Novi Sad, Serbia
We tested the efficacy of PreSens SensorPlugs for monitoring bacterial cultures in microfluidic bioreactors (MBs), which is important in the context of monitoring potential…
Download PDFDevelopment of Biomimetic Lung-on-a-Chip Platform: Oxygen transfer measurements with SensorPlugs at the inlet and outlet
Daniel Cheng, Charles Klassen, Harald C. Ott
IVIVA Medical, Beverly, MA, USA
The goal of this project was to provide a biomimetic lung-on-a-chip platform for tissue engineering and specifically for investigations of disease pathology. We first established…
Download PDFA Microfluidic Platform for Real-Time Non-Invasive Monitoring of Microglia Polarization: Application of novel optical SensorPlugs for microfluidics
Elifsu Polatli1,2, Alperen Yilmaz1,3, Resul Özbilgiç1,2, Íbrahim H. Erbay1,2, Duygu Sağ1,2,4, Sinan Güven1,2,4,*
1Izmir Biomedicine and Genome Center, Izmir, Turkey
2Dokuz Eylul University, Izmir International Biomedicine and Genome Institute, Izmir, Turkey
3Ankara University, Department of Biomedical Engineering, Izmir, Turkey
4Dokuz Eylul University, School of Medicine, Izmir, Turkey
*Corresponding author: sinan.guven@ibg.edu.tr
In this study, we hypothesized that sensor systems can be utilized for non-invasive real-time monitoring in microglia metabolism to further elucidate the polarization levels…
Download PDFOnline CO2 Monitoring in CHO Cell Culture: Bypass-pCO2 Measurements
Christoph Neubrand, Johannes Wirth, Dietmar Lang, Marvin Kadisch, Konstantin Bagnjuk
Rentschler Biopharma SE, Laupheim, Germany
A fed-batch-process with Horizon’s glutamine synthetase knockout CHO cell line was carried out in an autoclavable 10L benchtop bioreactor. For online culture monitoring PreSens’…
Download PDFInterpreting SFR vario Online Data Gathered in Plant Cell Suspension Culture: Characterization of plant cell lines for successful production processes
R. W. Maschke1, B. Pretzner2, G. T. John3, D. Eibl1
1Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Wädenswil, Switzerland
2Werum IT Solutions GmbH, Vienna, Austria
3PreSens Precision Sensing GmbH, Regensburg, Germany
Characterizing CHO Cell Lines with SFR vario: What online measured data can tell about your cell culture
R. W. Maschke1, B. Pretzner2, G. T. John3, D. Eibl1
1Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Wädenswil, Switzerland
2Werum IT Solutions GmbH, Vienna, Austria
3PreSens Precision Sensing GmbH, Regensburg, Germany
Cell Culture Monitoring in Stirred-Tank Bioreactor with Optical pH Sensors: Novel pH probe and pH sensor spots used in long-term cell culture
Melissa Hill1, Greg Laslo1, Marcelo Kern1, Sayantan Bose1, Christian Krause2
1GlaxoSmithKline, King of Prussia, PA, USA
2PreSens Precision Sensing GmbH, Regensburg, Germany
We used PreSens novel autoclavable optical sensor spots, as well as a novel pH probe, for online cell culture monitoring. Optical measurements were compared to reference data from…
Download PDFEvaluation of an Optical CO2 Probe for Long-Term Monitoring in Stirred-Tank Bioreactors: Prototype Autoclavable CO2 Sensor Used in Low and High Cell Density Cultures
Melissa Hill1, Marcelo Kern1, Sayantan Bose1, Athanas Apostolidis2
1GlaxoSmithKline, King of Prussia, PA, USA
2PreSens Precision Sensing GmbH, Regensburg, Germany
When culturing mammalian cells, carbon dioxide gas is an important factor to stabilize the pH in the medium and to adjust the atmosphere to physiological levels for the respective…
Download PDFEvaluation of an Optical O2 Probe and Sensor Spots for Long-Term Measurements in Stirred-Tank Bioreactors: Mammalian Cell Culture Monitoring with OXYPro® and Sensor Spots SP-PSt3
Melissa Hill1, Greg Laslo1, Marcelo Kern1, Sayantan Bose1, Christian Krause2
1GlaxoSmithKline, King of Prussia, PA, USA
2PreSens Precision Sensing GmbH, Regensburg, Germany
We used PreSens optical sensor spots and the OXYPro® oxygen probe for cell culture monitoring in a stirred-tank bioreactor. The optical measurements were compared to data…
Download PDFHow Much O2 Do Cells Really Face?: Continuous assessment of vertical O2 gradients in static adherent cell culture with the VisiSens™ MIOS system
L. Sauer1, D. Obermeier2, G. Liebsch2, R. J. Meier2, J. Wegener1,3
1Intitute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
This application note describes a method to continuously measure vertical oxygen gradients (VOGs) non-invasively in cell culture vessels using a cell culture imaging insert called…
Download PDFSpatio-temporal O2 Gradients in the Microenvironment of an Outgrowing Cell Patch: Oxygen Imaging in Micro-Scale with the VisiSens TD MIC System
C. Schmittlein1, R. J. Meier2, J. Wegener1,3
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
Cell-based assays have emerged to an indispensable tool in all areas of the biomedical sciences. However, cell culture experiments in a laboratory environment are always questioned…
Download PDFOxygen Consumption Rates of Adherent MDCK II Monolayer Cells: A Comparison of Open and Closed Systems Using the VisiSens TD Imaging System
C. Schmittlein1, R. J. Meier2, J. Wegener1
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Oxygen is a molecule of utmost importance for biological systems because it is necessary for life but may also induce detrimental damage. Thus, the local O2 concentration is an…
Download PDFIntegration of Chemical-Optical Oxygen and pH Sensors in Existing Control Units: Cultivation monitoring in a glass bioreactor system with subsequently integrated sensor spots
Cedric Schirmer1, Vivian Ott1, Gernot T. John2, Dieter Eibl1
1Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Institute of Chemistry and Biotechnology, Wädenswil, Switzerland
2PreSens Precision Sensing GmbH, Regensburg, Germany
The use of chemical-optical sensors for monitoring a cultivation using bioreactors with an already existing control unit requires the input of additional measuring signals. For…
Download PDFMonitoring Oxygenation in Microfluidic Cell Culture Using 2D Sensor Foils as Growth Substrate (full text): Oxygen Imaging with VisiSens™
C. Schmittlein1, R. J. Meier2, L. Sauer1, G. Liebsch2, J. Wegener1,3
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
White Paper Summary
Cells respond in very different manner regarding their microenvironment oxygenation. The oxygen availability for cells in microfluidics is strongly restricted…
Download PDFSFR vario Discriminates Good from Bad in Shake Flasks: Online Biomass and O2 measurements help optimize screening conditions
H. Morschett
Microbial Bioprocess Lab - Helmholtz Innovation Lab, Institute of Bio- and Geosciences, IBG-1: Biotechnology, Foschungszentrum Jülich GmbH, Germany
Shake Flasks are widely used for screenings of production strains. We used the SFR vario for simultaneous online monitoring of biomass and oxygen in GFP producing C. glutamicum…
Download PDFDirect Measurements of pCO2 in the Digestive Tract of Fish: Use of fiber optic pCO2 microsensors to reliably measure in vivo pCO2 in various sections of the gastrointestinal system in several fish species
Chris M. Wood1,2, Junho Eom1,2, Ellen H. Jung1,2, and Fernando Martinez Ferreras3
1Department of Zoology, University of British Columbia, Vancouver, B. C., Canada
2Bamfield Marine Sciences Centre, Bamfield, B. C., Canada
3PreSens Precision Sensing GmbH, Regensburg, Germany
Our objective was to measure the pCO2 levels in the fluids of the stomach and various sections of the intestine in both fasted and fed fish, weighing typically 50 - 300 g. It was…
Download PDFAdhesive Cell Growth and Microscopy on O2 and pH Sensor Foils: Impact of coating on the adhesion of cells upon VisiSens™ sensor foils SF-RPSu4 and SF-HP5R
C. Schmittlein1, R. J. Meier2, G. Liebsch2, J. Wegener1,3
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Regensburg, Germany
Suspension of Madin-Darby Canine Kidney cells (MDCK-II) were plated on VisiSens™ sensor foils SF-RPSu4 (O2) and SF-HP5R (pH) that were integrated in regular Petri dishes. Cell…
Download PDFUndisturbed Measurements of Oxygen in Deep Sea Sediments with Oxygen Sensitive Foil: The OXY-1 SMA system allows for fast measurements of pore water oxygenation in closed sediment cores
Patrick Blaser1, Jacek Raddatz2
1Institute of Earth Sciences, Heidelberg University, Heidelberg, Germany
2Insitute of Geosciences, Goethe University, Frankfurt, Germany
The transition from high to low and zero oxygen content in deep sea sediments provokes many important redox reactions in sedimentary pore waters, which are of high interest for…
Download PDFGas Input Measurements Using an Optical Oxygen Probe: Oxygen Input into the Liquid Phase of Three Different Reactor Concepts Measured with OXY-1 SMA trace
Natalie Mayer
Institute of Chemical Reaction Engineering, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany
The aim of this work was to show the performance of three different reactor concepts concerning the oxygen input into the liquid phase. For this purpose, water was aerated at 4.2…
Download PDFMonitoring in an Isolated Perfused Heart Test Stand with PreSens Optical Sensors: O2, CO2 and Glucose Flow-through Cells Integrated in a Blood Perfusion System
Daniela Obermaier1 & Giuliano Giacoppo2
1PreSens Precision Sensing GmbH, Regensburg, Germany
2Regensburg Center of Biomedical Engineering, University of Applied Sciences, Regensburg, Germany
During long-term application of artificial lungs (Extra Corporeal Membrane Oxygenation - ECMO) different complications, like blood clotting, cell destruction (hemolysis), or…
Download PDFImaging of pH and pO2 on Irradiated Fibroblasts and Oral Squamous Carcinoma Cells: Dual read-out of pH and pO2 in 96 well plates with VisiSens TD
Yvonne Reinders1, Robert J. Meier2, Gregor Liebsch2 and Frank Haubner3
1Department of Plastic, Aesthetic, Hand & Reconstructive Surgery, University Hospital Regensburg, Regensburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Department of Otorhinolaryngology, Ludwig-Maximilians-University Munich, Munich, Germany
O2, pH and CO2 Dynamics in Salt Marsh Tidal Ponds: Investigations under Different Light Regimes with Optical Profiling Microsensors and an Automated Micromanipulator
Ketil Koop-Jakobsen1 and Martin Gutbrod2
1University of Bremen, MARUM - Center for Marine Environmental Scienes, Bremen, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Salt marsh ponds are extreme environments characterized by high microbial activity and strong biogeochemical gradients at the sediment water interface. In this study, we…
Download PDFLight vs. Dark Oxygen Demand of Plankton Samples: O2 Measurements with SensorVials and the SDR SensorDish® Reader
Dr Sabine Schultes
Aquatic Ecology Group, LMU Munich, Germany
The dynamics of production and consumption of O2 in natural plankton communities change along environmental gradients and yield important information on the community ecology and…
Download PDFQuality Assessment of Primary Human Keratinocyte Cultures: Online Measurement of Oxygen and pH with the SDR SensorDish® Reader
V. Lorenz, B. Roski, A. Hermann, and H. F. Abts
Celonic GmbH, Jülich, Germany
The SDR SensorDish® Reader allows non-invasive analysis of dissolved oxygen (DO) and pH in multiwell plates. In this sutdy the sensitivity of the monitoring device was tested in…
Download PDFOptical Biomass Measurements in a Salvia fruticosa Cell Culture: Evaluation of the SFR vario for plant cell applications
P. Scherhag1, G. T. John2, C. Haas3 and J.-U. Ackermann1
1University of Applied Sciences, Department of Biochemistry and Bioengineering, Dresden, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
3Technical University, Institute fo Natural Materials Technology, Dresden, Germany
Suspension culture of plant cells is gaining importance in production of pharmaceutically active substances as it is independent from environmental influences. In order to…
Download PDFCO2, Oxygen and Biomass Monitoring in E. coli Shake Flask Culture: Following Glucose-Glycerin Diauxie Online with the SFR vario
Helga Tietgens1, Gernot T. John2 and Katja Bettenbrock1
1Max Planck Institut für Dynamik komplexer technischer Systeme, Magdeburg, Germany
2PreSens Precision Sensing GmbH, Regensburg, Germany
Diauxic growth of E. coli in medium containing glycerin and glucose was monitored online with the SFR vario. The device measures biomass online via scattered light detection.…
Download PDFOxygen Gradients in Collagen-I Gels: Automated micromanipulator with fiber optic profiling oxygen microsensor as a method to evaluate oxygen gradients in collagen-I gels for tissue engineering
Laura Heimann1, Paul Wolff1, Elizabeth Rosado Balmayor1, Martijn van Griensven1, Gregor Liebsch2, Robert Meier2, Martin Gutbrod2
1Experimental Trauma Surgery, Technical University of Munich (TUM), Munich, Germany
2PreSens GmbH, Regensburg, Germany
Adipose-derived mesenchymal stem cell loaded collagen-I gels were evaluated with the automated profiling system by PreSens (Automated Micromanipulator AM and Profiling O2…
Download PDFOxygen Consumption of Crayfish under Different Temperature Regimes: Monitoring with oxygen microsensors and the OXY-4 micro
Tanja Soukup, Joachim Henjes, and Matthew James Slater
Alfred Wegener Institute, Helmholtz Centre for Polar- and Marine Research (AWI), Bremerhaven, Germany
The narrow clawed crayfish is able to withstand increased temperatures (≥ 26 °C) for medium term periods, but it is still unknown what happens to the organism during the exposure…
Download PDFSimultaneous pH, CO2 and O2 Measurements in Algae Photobioreactor: pH Flow-Through Cells successfully evaluated for later use in the International Space Station ISS
Gisela Detrell and Jochen Keppler
Institute of Space Systems, University of Stuttgart, Germany
At the Institute of Space Systems several experiments with algae photobioreactors were conducted, in order to design a system to be tested in the International Space Station in…
Download PDFOnline Monitoring of S. cerevisiae Culture with the SFR vario: New online biomass data is in very good accordance with offline measurements
Dylan Haskew1, Gernot T. John2, and Dogan Ornek1
1Upstream Process Development, FUJIFILM Diosynth Biotechnologies U. S. A., Inc., US
2PreSens Precision Sensing GmbH, Germany
In order to investigate a model process with Saccharomyces cerevisiae the new SFR vario shake flask reader was used to monitor O2, OUR, pH, and biomass online inside 2 L shake…
Download PDFNon-invasive Online CO2 Measurement with Chemical Optical CO2 Sensors: Chemical optical CO2 sensors provide a versatile tool for online monitoring of dissolved CO2 in various scale and designs
Athanas Apostolidis
PreSens Precision Sensing GmbH, 93053 Regensburg, Germany
Accurate CO2 measurement in the liquid phase has been a challenge for decades. Although off-gas measurement by means of IR-spectroscopy is a proven technology, the correlation of…
Download PDFThyroid-on-a-Chip: An Organoid Platform for In Vitro Assessment of Endocrine Disruption
Daniel J. Carvalho, Anna M. Kip, Mírian Romitti, Marta Nazzari, Andreas Tegel, Matthias Stich, Christian Krause, Florian Caiment, Sabine Costagliola, Lorenzo Moroni, and Stefan Giselbrecht
Adv. Healthcare Mater. (2023) 2201555
Elevated micro-topography boosts growth rates in Salicornia procumbens by amplifying a tidally-driven oxygen pump: implications for natural recruitment and restoration
Gregory S. Fivash, Jim van Belzen, Ralph J. M. Temmink, Karin Didderen, Wouter Lengkeek, Tjisse van der Heide, Tjeerd J. Bouma
Annals of Botany (2019)
Tomato plants rather than fertilizers drive microbial community structure in horticultural growing media
Oliver Grunert, Ana A. Robles-Aguilar, Emma Hernandez-Sanabria, Silvia D. Schrey, Dirk Reheul, Marie-Christine Van Labeke, Siegfried E. Vlaeminck, Tom G. L. Vandekerckhove, Mohamed Mysara, Pieter Monsieurs, Vicky M. Temperton, Nico Boon & Nicolai D. Jablonowski
Scientific Reports (2019) 9:9561
Regulation of Primary Metabolism in Response to Low Oxygen Availability as Revealed by Carbon and Nitrogen Isotope Redistribution
Carla António, Carola Päpke, Marcio Rocha, Houssein Diab, Anis M. Limami, Toshihiro Obata, Alisdair R. Fernie, and Joost T. van Dongen
Plant Physiology (2016), Vol. 170, 43 - 56
Hyaluronic acid facilitates chondrogenesis and matrix deposition of human adipose derived mesenchymal stem cells and human chondrocytes co-cultures
Elisabeth Amann, Paul Wolff, Ernst Breel, Martijn van Griensven, Elizabeth R. Balmayor
Acta Biomaterialia 52 (2017) 130 - 144
A microfluidic oxygen sink to create a targeted cellular hypoxic microenvironment under ambient atmospheric conditions
Samineh Barmaki, Ville Jokinen, Daniela Obermaier, Daria Blokhina, Matti Korhonen, Robin H.A. Ras, Jyrki Vuola, Sami Franssila, Esko Kankuri
Acta Biomaterialia (2018)