Find Publications and Information about Your Optical Sensor System
Whether it is general information or detailed, application based data on optical sensors you are looking for, in our collection of publications, sensor basics and FAQs you will find what you need. Furthermore, short tutorial videos and our instruction manuals will help you with handling your sensor system.
O2-sensitive Microcavity Arrays for 3D Cell Culture Monitoring: Oxygen Imaging in Spheroids with VisiSens TD Technology
Microthermoforming is applied to create an array of microcavities in oxygen sensor foils. 3D cell aggregates can be cultured in these cavities and the oxygen content can be…
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Online Monitoring of C. glutamicum Culture in Ultra Yield® Flasks: SFR vario Used with Only Slightly Translucent Shake Flasks
In this study we analyzed, if slightly translucent polypropylene shake flasks can be used for optical measurements and especially optical biomass monitoring with the SFR vario. As…
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Why It Is Important to Measure Different Parameters Online in Shake Flasks: SFR vario Measures O2, OUR, pH and Biomass Online
Optical sensors enable monitoring and controlling important culture parameters even at shake flask scale. In this short report we show an example of multiparameter monitoring with…
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In situ Remediation of Contaminated Groundwater: VisiSens TD used to study release of groundwater remedial amendments from well screens under flow-through conditions
This application note shows how the VisiSens imaging technology can be used to visualize and quantify the release of groundwater remedial amendments from unpumped wells under…
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Fungal Metabolite Screening Inside a Microfluidic Bioreactor: pH Measurements in Plant Cell Culture with pH-1 SMA HP5
As sessile organisms, plants cannot easily circumvent stressors by relocating. To evaluate the quality and quantity of abiotic and biotic stresses experienced, plants have evolved…
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Testing Plant Seed Viability by Respiration Measurements: Oxygen Consumption Monitoring in 96 Well Plate with VisiSens TD and ViviPlate a Newly Developed Airtight Plate Clamp System
PreSens has developed the sensor and clamp tool ViviPlate that allows to create closed respirator chambers of different numbers and volumes in microtiterplate format. These…
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O2, pH and CO2 Monitoring in Microfluidic Mammalian Cell Culture: Cultivated Meat Bioprocess Optimization with SensorPlugs
We tested the efficacy of PreSens SensorPlugs for monitoring mammalian cell culturing processes in microfluidic bioreactors (MBs) with integrated sensors. MRC-5 fibroblasts (ATCC®…
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Development of Biomimetic Lung-on-a-Chip Platform: Oxygen transfer measurements with SensorPlugs at the inlet and outlet
The goal of this project was to provide a biomimetic lung-on-a-chip platform for tissue engineering and specifically for investigations of disease pathology. We first established…
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A Microfluidic Platform for Real-Time Non-Invasive Monitoring of Microglia Polarization: Application of novel optical SensorPlugs for microfluidics
In this study, we hypothesized that sensor systems can be utilized for non-invasive real-time monitoring in microglia metabolism to further elucidate the polarization levels…
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Cell Culture Monitoring in Stirred-Tank Bioreactor with Optical pH Sensors: Novel pH probe and pH sensor spots used in long-term cell culture
We used PreSens novel autoclavable optical sensor spots, as well as a novel pH probe, for online cell culture monitoring. Optical measurements were compared to reference data from…
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Evaluation of an Optical CO2 Probe for Long-Term Monitoring in Stirred-Tank Bioreactors: Prototype Autoclavable CO2 Sensor Used in Low and High Cell Density Cultures
When culturing mammalian cells, carbon dioxide gas is an important factor to stabilize the pH in the medium and to adjust the atmosphere to physiological levels for the respective…
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Evaluation of an Optical O2 Probe and Sensor Spots for Long-Term Measurements in Stirred-Tank Bioreactors: Mammalian Cell Culture Monitoring with OXYPro® and Sensor Spots SP-PSt3
We used PreSens optical sensor spots and the OXYPro® oxygen probe for cell culture monitoring in a stirred-tank bioreactor. The optical measurements were compared to data…
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How Much O2 Do Cells Really Face?: Continuous assessment of vertical O2 gradients in static adherent cell culture with the VisiSens™ MIOS system
This application note describes a method to continuously measure vertical oxygen gradients (VOGs) non-invasively in cell culture vessels using a cell culture imaging insert called…
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Measuring Leaf O2 Consumption Rates with the SDR® SensorDish Reader: Adapted SDR Set-Up with Tubes and O2 Sensor Spots in Screw Caps
I tested whether the influence of external metabolites on leaf O2 consumption rates could be measured with the SDR. Specifically, it was previously observed in a screen of…
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Spatio-temporal O2 Gradients in the Microenvironment of an Outgrowing Cell Patch: Oxygen Imaging in Micro-Scale with the VisiSens TD MIC System
Cell-based assays have emerged to an indispensable tool in all areas of the biomedical sciences. However, cell culture experiments in a laboratory environment are always questioned…
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Oxygen Consumption Rates of Adherent MDCK II Monolayer Cells: A Comparison of Open and Closed Systems Using the VisiSens TD Imaging System
Oxygen is a molecule of utmost importance for biological systems because it is necessary for life but may also induce detrimental damage. Thus, the local O2 concentration is an…
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Integration of Chemical-Optical Oxygen and pH Sensors in Existing Control Units: Cultivation monitoring in a glass bioreactor system with subsequently integrated sensor spots
The use of chemical-optical sensors for monitoring a cultivation using bioreactors with an already existing control unit requires the input of additional measuring signals. For…
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Monitoring Oxygenation in Microfluidic Cell Culture Using 2D Sensor Foils as Growth Substrate (full text): Oxygen Imaging with VisiSens™
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Cells respond in very different manner regarding their microenvironment oxygenation. The oxygen availability for cells in microfluidics is strongly restricted…
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SFR vario Discriminates Good from Bad in Shake Flasks: Online Biomass and O2 measurements help optimize screening conditions
Shake Flasks are widely used for screenings of production strains. We used the SFR vario for simultaneous online monitoring of biomass and oxygen in GFP producing C. glutamicum…
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Quantification of Oxygen Consumption of Meat-Spoiling Microorganisms below 30 % CO2 / 70 % O2 Atmosphere: Measurements in a Simulated Meat Packaging Environment with Optical Oxygen Sensor Spots SP-PSt3
In order for meat products to maintain their red color, beef and pork are packaged under a modified atmosphere. Mainly, a protective gas atmosphere consisting of 30 % CO2 and 70 %…
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Direct Measurements of pCO2 in the Digestive Tract of Fish: Use of fiber optic pCO2 microsensors to reliably measure in vivo pCO2 in various sections of the gastrointestinal system in several fish species
Our objective was to measure the pCO2 levels in the fluids of the stomach and various sections of the intestine in both fasted and fed fish, weighing typically 50 - 300 g. It was…
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Adhesive Cell Growth and Microscopy on O2 and pH Sensor Foils: Impact of coating on the adhesion of cells upon VisiSens™ sensor foils SF-RPSu4 and SF-HP5R
Suspension of Madin-Darby Canine Kidney cells (MDCK-II) were plated on VisiSens™ sensor foils SF-RPSu4 (O2) and SF-HP5R (pH) that were integrated in regular Petri dishes. Cell…
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Undisturbed Measurements of Oxygen in Deep Sea Sediments with Oxygen Sensitive Foil: The OXY-1 SMA system allows for fast measurements of pore water oxygenation in closed sediment cores
The transition from high to low and zero oxygen content in deep sea sediments provokes many important redox reactions in sedimentary pore waters, which are of high interest for…
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Non-invasive pH Monitoring in Anaerobic Clostridium Culture: Evaluation of the pH Sensor Spot SP-LG1 in Low pH Range
A prototype pH sensor spot type LG1 was integrated in a closed, gas-tight culture bottle for monitoring an anaerobic cultivation of Clostridium acetobutylicum. The sensor allowed…
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Gas Input Measurements Using an Optical Oxygen Probe: Oxygen Input into the Liquid Phase of Three Different Reactor Concepts Measured with OXY-1 SMA trace
The aim of this work was to show the performance of three different reactor concepts concerning the oxygen input into the liquid phase. For this purpose, water was aerated at 4.2…
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Monitoring in an Isolated Perfused Heart Test Stand with PreSens Optical Sensors: O2, CO2 and Glucose Flow-through Cells Integrated in a Blood Perfusion System
During long-term application of artificial lungs (Extra Corporeal Membrane Oxygenation - ECMO) different complications, like blood clotting, cell destruction (hemolysis), or…
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High Resolution O2 Microprofiling of a Freshwater Stream Biofilm: Use of an Automated Micromanipulator System and O2 Microsensors in a Flume Experiment
The objective of this study was to determine oxygen fluxes and the spatial micro-heterogeneity of microbial respiration and photosynthesis of a stream biofilm from the Selke…
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Oxygen Measurements of Innate Immune Cells in Suspension Cultures: In vitro Monitoring with Fibox 4 and Optical O2 Sensor Spots
Here, we describe data on the oxygen measurements in suspension cultures of innate immune cells: Primary murine bone marrow-derived mast cells and primary human blood-derived…
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In vivo Monitoring of Haemolymph pCO2 in Dragonfly Nymphs to Determine the Resting State Value: Use of fiber optic pCO2 Microsensors to reliably measure in vivo haemolymph pCO2 in insects
The objective of this study was to monitor the haemolymph pCO2 of water-breathing dragonfly nymphs in vivo. However, the small body size and haemolymph volume of these animals…
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O2, pH and CO2 Dynamics in Salt Marsh Tidal Ponds: Investigations under Different Light Regimes with Optical Profiling Microsensors and an Automated Micromanipulator
Salt marsh ponds are extreme environments characterized by high microbial activity and strong biogeochemical gradients at the sediment water interface. In this study, we…
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Light vs. Dark Oxygen Demand of Plankton Samples: O2 Measurements with SensorVials and the SDR SensorDish® Reader
The dynamics of production and consumption of O2 in natural plankton communities change along environmental gradients and yield important information on the community ecology and…
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Revealing Oxygen Dependency of Bioluminescent TNT-Sensing E. coli K12 MG1655: High Resolution Oxygen Measurements with a Bare Fiber Optical Microsensor and OXY-1 ST
Buried explosive material containing TNT represents a severe issue for human and animal safety. Its additional high toxic potential and harmful effects to the environment manifest…
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Measuring Dissolved O2 in Dimethyl Sulfoxide Solution in the Presence of Different Lithium Salts: Investigations on organic electrolytes with the OXY-1 SMA and optical O2 Sensor Spots
In Li-O2 batteries the concentration of dissolved oxygen in the organic electrolyte is a very important parameter. As being a reactant in the reduction reaction, its concentration…
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Quality Assessment of Primary Human Keratinocyte Cultures: Online Measurement of Oxygen and pH with the SDR SensorDish® Reader
The SDR SensorDish® Reader allows non-invasive analysis of dissolved oxygen (DO) and pH in multiwell plates. In this sutdy the sensitivity of the monitoring device was tested in…
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Optical Biomass Measurements in a Salvia fruticosa Cell Culture: Evaluation of the SFR vario for plant cell applications
Suspension culture of plant cells is gaining importance in production of pharmaceutically active substances as it is independent from environmental influences. In order to…
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The Application of the SFR Shake Flask Reader in Teaching at the Esslingen University of Applied Sciences: Non-invasive Measurements of Dissolved Oxygen and pH in Shake Flasks within the Framework of Practical Courses and Student Projects
Dissolved oxygen (DO) and pH are key parameters for microbial fermentations and cell cultures. The importance of DO and pH for bioprocesses as well as methods for their measurement…
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CO2, Oxygen and Biomass Monitoring in E. coli Shake Flask Culture: Following Glucose-Glycerin Diauxie Online with the SFR vario
Diauxic growth of E. coli in medium containing glycerin and glucose was monitored online with the SFR vario. The device measures biomass online via scattered light detection.…
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Oxygen Gradients in Collagen-I Gels: Automated micromanipulator with fiber optic profiling oxygen microsensor as a method to evaluate oxygen gradients in collagen-I gels for tissue engineering
Adipose-derived mesenchymal stem cell loaded collagen-I gels were evaluated with the automated profiling system by PreSens (Automated Micromanipulator AM and Profiling O2…
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Oxygen Consumption of Crayfish under Different Temperature Regimes: Monitoring with oxygen microsensors and the OXY-4 micro
The narrow clawed crayfish is able to withstand increased temperatures (≥ 26 °C) for medium term periods, but it is still unknown what happens to the organism during the exposure…
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Simultaneous pH, CO2 and O2 Measurements in Algae Photobioreactor: pH Flow-Through Cells successfully evaluated for later use in the International Space Station ISS
At the Institute of Space Systems several experiments with algae photobioreactors were conducted, in order to design a system to be tested in the International Space Station in…
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Oxygen Dynamics around Buried Tar Balls in Florida Marine Sands: A laboratory flume study testing the VisiSensTM oxygen imaging system
After the Deepwater Horizon accident in 2011, crude oil in unknown amounts was embedded in sandy shelf and shore sediments of the northeastern Gulf of Mexico. Oxygen is a key…
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Perfusion Monitoring in Microvascular Flaps: Fluorescence Imaging Applied as a Method to Discover Vessel Thrombosis
Tissue transfer in head and neck reconstruction has a high success rate. Nevertheless, in up to 10 % of all cases serious complications occur. Skin flap monitoring with technical…
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Measuring Respiration and Primary Production Dynamics in Macroalgae: 2D imaging of oxygen gradients in F. vesiculosus with the VisiSens system
In this study the VisiSens system was used for 2D imaging of small scale differences in primary production and respiration in macroalgae. F. vesiculosus was used as a model…
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Viability of Lithobiontic Microorganisms Inhabiting the Rocks of Atacama Desert: Determining photosynthetic activity with VisiSens
The hyper-arid, life-limited Atacama Desert (Chile) gives the opportunity to determine the potential for life in extraterrestrial environments. In this study the viability of…
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Oxygen Dynamics in the Capillary Fringe: Mapping oxygen consumption of Pseudomonas fluorescens with VisiSens
The capillary fringe is a highly active zone for chemical and biological transformation at the transition of the vadose zone and groundwater. To study aerobic biodegradation in the…
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Recording Spatial Patterns of Oxygen Consumption in Individual Corals: Assessing Coral Health Under a Changing Climate with VisiSens
The calcified skeleton of corals makes it difficult to take biological measurements. The VisiSens imaging system was applied to measure oxygen consumption patterns in individual…
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Effect of Non-Symbiotic Hemoglobin Expression on Oxygen Content in Roots: Oxygen Imaging and Respiration Measurement in Roots with VisiSens
This project aimed to define the role of plant non-symbiotic hemoglobin and regulation of respiration by nitric oxide in plant roots. Wild-type and hemoglobin over-expressing…
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Effect of Pseudomonas Infection on Oxygen Status of Arabidopsis Leaves: Visualizing Oxygen Dynamics in Plant Tissue
In order to prevent pathogens from spreading, infected plants use a form of cell death - the so called hypersensitive response. In this study the widely used model system of Arabid…
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Monitoring Oxygen in a Mouse Model of Renovascular Hypertension: Checking Microvascular Perfusion in Mouse Kidney with the VisiSens System
In this study we used a mouse model of renovascular hypertension known as the two-kidney, one clip model (2K1C). In this model one renal artery is constricted which ultimately…
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Benthic Disturbance-Recovery Dynamics in a Changing Coastal Ocean: Imaging the effects of terrestrial sediment deposits on intertidal sandflat pH and O2 with VisiSens technology
Changes in climate and land use increase the supply of terrestrial sediment to coastal water worldwide but the effects of this increase on ecosystem functioning are not well known.…
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Exchange of O2 and CO2 in the Capillary Fringe of a Porous Medium: A laboratory study testing the VisiSens imaging systems
Diffusion experiments were performed in a quartz sand packing to study the effect of evaporative conditions on mass transfer of oxygen and CO2 across the interface between the…
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Role of Oak Wood Ellagitannins on the Oxygen Transfer Rate in Wine Barrels: 2D Visualization of Oxygen Permeation with VisiSens
Oxygen permeation through oak wood in a wine barrel scenario has been recorded with the VisiSens™ oxygen imaging system. A special measurement chamber was designed to simulate…
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Imaging the Oxygen Consumption of Microbial Cultures: Metabolic activity of E. coli monitored inside the incubator with VisiSens A1
Oxygen imaging with VisiSens™ A1 allows 2-dimensional mapping of oxygen consumption inside microbial culture. The compact measurement system can be applied for monitoring the…
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Monitoring pO2 in Cell Culture to Improve Directed Differentiation: Visualizing oxygen distributions in 6 well plates with VisiSens A1
Tissue oxygenation during in vitro culture of stem cells is a critical factor. Previous studies have shown that maintaining pO2 during in vitro culture at levels approximating the…
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Imaging Rhizosphere pH and CO2 Dynamics: Plant Root - Soil Interactions Quantified with Prototype VisiSens Systems
Prototype VisiSens™ devices for pH & CO2 imaging were used to perform continuous and highly resolved measurements in rhizobox systems. pH was measured around durum wheat roots…
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Extracorporeal Membrane Oxygenator to Support Diseased Lungs: Monitoring the gas exchange capacity of hollow fibers with VisiSens
The function of a diseased lung can be supported by the application of an extracorporeal membrane oxygenator (ECMO). Aim of this study is to improve the hemocompatibility of the…
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Assessment of Oxygen Depletion and Biofilm Structure Grown in MBBR Carriers: Tracking the Distribution of Oxygen Penetration through Biofilm Carriers with VisiSens
Biofilm systems like moving bed biofilm reactors (MBBR) are used for wastewater treatment. Preliminary tests were conducted to evaluate the suitability of 2D oxygen imaging for…
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Measuring Oxygen Gradients above a Respiratory Active Algal Cell Layer: VisiSens Applied to Detect Dynamic Oxygen Fluctuations over Time
Oxygen gradients in buffer solution above a monolayer of Chlamydomonas reinhardtii have been visualized with the VisiSens system. Aim of the experiment was to determine, if…
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Immobilized Plant Cells - From DSMZ to the Customer: VisiSens technology used to detect metabolic activities of immobilized plant cells during simulated cell transport in a closed system
The DSMZ is one of the largest biological resource centers worldwide. Its collections currently comprise almost 40,000 items, including about 700 undifferentiated plant cell lines.…
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Diffusive / Dispersive Reaction Fronts in Groundwater: 2D visualization of oxygen and pH using the VisiSens imaging system to study Fe2+ oxidation under flow-through conditions
Quasi 2D flow-through experiments were performed in heterogeneous porous media to study the oxidation of ferrous iron in groundwater using the VisiSens™ imaging system for oxygen…
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Biogenic Gas Bubbles Produced within Soil Macropores: Simultaneous measurement of gas bubble size and CO2 concentration with VisiSens A3
Greenhouse gas measurements in wetlands note considerable spatial and temporal variability in biogenic gas bubbles, suggesting microscale heterogeneities influence formation and…
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Integration of Oxygen Sensor Foils in Microfluidic Chips: Applicability of VisiSens in Micro-Channels made by Ultrasonic Welding
We evaluated the VisiSens™ A1 imaging system for its applicability in micro-channels to create a microfluidic chip with integrated oxygen sensing. The experiments described here…
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Assessing Biocide Actions on Lichen with VisiSens: Controlling microbial biodeterioration on a dolostone Roman bridge
Historical monuments are often subject to microbial bio deterioration. Effective treatments that inhibit biological growth on ancient rock surfaces have to be found. In this study…
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O2, CO2 and pH Dynamics in the Capillary Fringe: VisiSens combined with an automated linear positioning system provides complete analyte maps
We investigated the biochemical transformation potential in the capillary fringe (CF) by measuring the distribution of dissolved gases and the pH value. Therefore, we combined the…
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Optical pH Surveillance in a Microfluidic System for Cell Culture: Evaluation of VisiSens pH sensor foils integrated in microfluidic systems
pH surveillance within microfluidic systems is a challenging problem. Liquid quantities available for measurement are limited and also relatively inaccessible within the…
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Development of a Partly Controllable System at Shake Flask Scale: Enhanced data quality obtained from fed-batch shake flask cultivation by minimizing disturbance variables monitored by the SFR
The main objective of this study was to develop an easy to handle and partly controllable process cultivation system at shake flask scale. First, it was investigated how…
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Investigation on ATP Synthesis via Oxidative Phosphorylation in C. glutamicum: Measurement of oxygen consumption by an ATP synthase deletion mutant and the wild type strain with the SFR Shake Flask Reader
A F1F0-ATP synthase deletion mutant of C. glutamicum was characterized and investigated in respect to growth, by-product formation and bioenergetics. The mutant did not grow with…
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Online Monitoring of Glucose, pH, and DO in Shake Flask Culture: Novel Glucose Sensor Used in Combination with the SFR Shake Flask Reader
A new single-use glucose sensor in combination with chemical optical oxygen and pH sensors was used for online monitoring of CHO cell culture in shake flasks. During the whole…
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Development of a Shaken Scale-Down Model: Characterization of Critical Parameters in Bioprocess Development
A shaken scale-down model at approx. 0.1 L scale for a previously established stirred-tank model at 2 L scale was developed. In the shaken scale-down model online monitoring of pH…
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Characterization of Microbial Growth Profiles: Online detection of oxygen and pH during culture in shake flasks
Shake flasks are the most commonly used cultivation systems for process and media optimization, screening for new products and strains as well as for the supply of inoculum for…
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Insect Cell Cultivation in Orbitally Shaken Flasks with Sensors: Oxygen mass transfer in insect cell-based high cell density cultivations
Insect cell-based processes have become increasingly influential for tool protein and Virus-like Particle (VLP) vaccine productions over the last years. At small scale insect cells…
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A New Method for Predicting Oxygen Supply during Aerobic Growth: Measurements with the SFR Shake Flask Reader allow kLa determination and reliable design of culture conditions in disposable shake flasks
Dissolved oxygen (DO) is a key parameter during aerobic cultivation and due to its low solubility oxygen can become limiting under certain culture conditions. In this study a new…
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New Device for Biomass Monitoring in Shake Flask Culture: Development of an analytical unit based on the SFR Shake Flask Reader
We developed a novel system for online biomass measurement in shake flasks based on near 360° scattered light detection. The optosensoric components were combined with the…
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Online Monitoring of S. cerevisiae Culture with the SFR vario: New online biomass data is in very good accordance with offline measurements
In order to investigate a model process with Saccharomyces cerevisiae the new SFR vario shake flask reader was used to monitor O2, OUR, pH, and biomass online inside 2 L shake…
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pH Monitoring in Dairy Starter Cultures: Characterization of acid production with optical sensors in microtiter plates
Starter cultures are used to guarantee good and reproducible quality of dairy products. In this study an easy-to-use method to screen a large number of inoculum cultures as quality…
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Oxygen and pH Monitoring in Deep Well OxoDishes® and HydroDishes®: Assessing optimal growth conditions for shaken yeast culture
In this study different yeast cultivations in deep well OxoDishes® and HydroDishes® were examined. Cultivations were carried out at varying volumes to assess optimal growth…
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Localized Oxygen Depletion by Transmigrating Neutrophils Influences Inflammatory Resolution: Oxygen monitoring in cell-cell cross talk experiments with the SDR SensorDish® Reader
Recent research on intestinal inflammation has shown that mucosal metabolism influences disease outcome. Intestinal inflammation is accompanied by accumulation of neutrophils…
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Correcting for Oxygen Diffusion in Respiration Measurements: Determining instantaneous oxygen consumption rate in OxoDishes® applied as closed-system respirometers
When using multiwell plates made of polysytrene - like the PreSens OxoDishes® - as closed-system respirometers, one has to take oxygen diffusion through the well material into…
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High Throughput Respiration Assay for Cytotoxicity Determination: A Method for the Evaluation of New Active Pharmaceutical Ingredients Using the SDR SensorDish® Reader
The objective of this project was to develop a cytotoxicity assay with high throughput by monitoring the cellular respiration in cell culture. Cells were cultured in 24-well plates…
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Co-Cultures of Caco-2 cells and E.coli: Optosensoric online monitoring of O2 uptake and extracellular acidification
Oligogalacturonic acids (OGAs), obtained by hydrolysis of pectin, are known to inhibit the adhesion of pathogenic bacteria on intestinal cells and thus to prevent infectious…
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Online Oxygen Monitoring in Cell Culture: Effects of mitochondrial modulators on O2 dynamics of mammalian cells
Real-time measurements of oxygen and pH in mammalian cell cultures are important to understand the metabolic dynamics in cell cultures. The SDR SensorDish® Reader allows the time…
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Energy Catabolism Measurements in Metastatic Macrophage M3 Cells: Cell respiration measurements with the SDR SensorDish Reader and the XF 96 Extracellular Flux Analyzer
In order to study the role of mycoplasma infection on cancer cell energy catabolism, we measured oxygen consumption and lactate production in either infected or non-infected…
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Design of Experiment (DoE) for Fed-batch Process Development in Shaken Cultures: Determination of reproducibility and scalability in Escherichia coli cultures grown under fed-batch conditions using SensorDishes® and Sensor Flasks
When designing a recombinant protein production process, a high number of parallel cultivations need to be carried out. This is mostly performed using batch cultures in shake…
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Evaluating the SDR SensorDish® Reader for Strain Development: Monitoring different E. coli strains inside deep well OxoDishes®
We conducted several tests to evaluate the applicability of the SDR SensorDish® Reader combined with deep well OxoDishes® in strain development. We measured oxygen consumption of…
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Identifying Water Oxidation Catalysts for Clean Fuel Production: High throughput screening of water oxidation reactions with OxoDishes®
The splitting of water in a light-driven reaction is one of the most promising processes for producing clean fuel. In order to create an efficient process suitable reaction…
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Simultaneous Monitoring of pH and Oxygenation in Microvolumes of Blood: Fiber optical pH microsensors and miniature broad range spectrophotometry advance blood function analysis
The implementation of a pH microsensor and a miniature broad range spectrophotometer in a gas diffusion chamber facilitated the pH dependent analysis of blood oxygen binding…
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pH Monitoring of Urine and Tumor Microenvironments in Rats: High Spatial Resolution Measurements Controlled with the Manual Micromanipulator
The aim of our study was to monitor pH of urine samples (< 100 µL) and of tumor microenvironments of anesthetized rats in a minimal invasive way. The small urine volumes of rats or…
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New mL-Scale Bioreactor for the Cultivation of Mycelium Forming Microorganisms: Determination of the Volumetric Oxygen Transfer Coefficient (kLa)
A new milliliter-scale stirred tank bioreactor was developed for the cultivation of mycelium forming microorganisms. Applying a newly designed one-sided paddle impeller an enhanced…
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Hypoxic Preconditioning of Human Mesenchymal Stem Cells: Restoring the Differentiation Potential of hMSCs under Hypoxic Conditions
In this study the osteogenic differentiation potential of hMSCs under hypoxic conditions of 2 % O2 in comparison to standard tissue culture oxygen atmosphere of 21 % was…
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Alginate-based Bi-layered Hydrogels with Embedded Cells for the Regenerative Therapy of Osteo-chondral Defects: Using needle-type oxygen microsensors to measure oxygen saturation in an artificial cartilage construct
For the translation of the tissue engineering approach to clinical applications, cell vitality is of crucial importance for the regeneration of impaired tissue. Insight in the…
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Hypoxia in Static and Dynamic 3D Culture Systems: Oxygen Gradient Measurements in 3D Culture Systems Designed for Bone Tissue Engineering
Gradients in tissue quality from the periphery towards the center are a limiting factor in tissue engineering of sizeable cell-scaffolds. Uneven oxygen supply might be the cause…
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pH Regulation for a Liver Cell Bioreactor: Prototype development with a chemical optical pH flow-through cell
An important aspect of efficient liver cell bioreactors is the automated regulation of physio-chemical culture parameters. The present study describes the development of the first…
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Assessing the Impact of pH Fluxes between the Sediment-Water Interface on the Overlaying Water Column off Namibia: Spatial variability of pore water pH investigated with single-use flow-through cells pH
Eastern boundary upwelling systems, such as the Benguela upwelling system off the south west African coast, are characterized by high concentrations of dissolved inorganic carbon…
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Effect of Oxygen Depletion on GDH Activity: Oxygen Monitoring in Shaken Cultures of C. glutamicum and C. diphtheriae
The impact of oxygen depletion in cultures of Corynebacterium glutamicum and two different strains of Corynebacterium diphtheriae was investigated under different cultivation…
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Validation Study of the Optrode Dual™: Oxygen and pH Measurements in a Single-Use Bioreactor with Integrated Optical Sensors Using a Conventional Controller
The Optrode Dual™ converts measurement data of optical sensors into electrochemical signals (ECS) which can be transferred to controller units for bioreactors. The user can record…
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Development of a New, Highly Sensitive Sensor for Permeation Measurement: Optical Sensors Used for Permeation Testing of High Barrier Materials
A new measurement system for testing synthetic materials on oxygen permeability was developed. Unlike the currently used systems with electrochemical or barometrical sensors it was…
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Constant Oxygen Monitoring during 13 Days on Satellite Mission in Outer Space: PreSens OEM Components Applied for Biological Experiments in Zero Gravity
The OMEGAHAB is a closed artificial ecosystem that was sent into orbit for 13 days on board the Russian satellite FOTON-M3 in 2007. Objective of this biological experiment was to…
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Perfusion Culture of Cell-seeded 3D Scaffolds for Tissue Engineering: In-line Oxygen Measurement in Perfusion Bioreactors
Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before…
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Integration of a PreSens Oxygen Sensor Spot Into a Solid Mini Bioreactor: Validation of a Newly Designed Sensor Adapter
This report illustrates the operation of a sensor adapter using PreSens sensor technology to measure environmental conditions inside a non-transparent solid mini bioreactor. With…
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Feedback Controlled Carbon Feeding at Shake Flask Scale: Online measurement of DO with chemical optical sensors by PreSens
In this study a feedback controlled scale-down model at shake flask scale has been developed. Dissolved oxygen (DO) concentration in cultures of Saccharomyces cerevisiae was…
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Determining the Impact of Shoreline Retrogressive Thermokarst Slumping to Tundra Lakes: Biological Oxygen Demand Measured with the Fibox 3 LCD trace
Permafrost temperatures in the high Arctic have been rising causing a deepening of the active layer and an increase in thermokarst activity. Permafrost degradation, often occurring…
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Optrode Dual Connects Single-Use Bioreactors to Conventional Controllers: Monitoring of CHO Cell Cultivation with Chemical Optical Sensors
The Optrode Dual™ prototype has been applied for measurements in suspension adapted CHO cell culture. The transmitter converts the reading of chemical optical sensors integrated in…
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Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels: Optical oxygen sensor spots help optimizing culture conditions
Optical measurement of oxygen allows the introduction of small sensor spots into the culture vessels. As many of the conventional sub-bioreactor scale culture vessels are not…
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Monitoring Oxygen Dynamics at the Sediment-Water Interface: Non-Invasive Measurements at Distinct Sites in SensorVials
In marine ecosystems processes at the sediment-water interface are influenced by the presence of an active benthic community living in the uppermost millimeters of the sediment. In…
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Online pH and DO Measurements in Microcarrier-Based hMSC Cultivations: Culture monitoring with sensor spots integrated in single-use spinner flasks
Spinner flasks are often used for microcarrier-based cultivations of human mesenchymal stem cells (hMSCs). Normally, they are not equipped with pH and dissolved oxygen (DO) probes.…
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Studying Microbial Iron and Manganese Oxidation in Different Experimental Set-Ups: Non-invasive oxygen monitoring using a Fibox 3, OXY-4 mini and oxygen sensitive sensor foils
At circumneutral pH three physiological types of microbial iron oxidation can occur, i. e. microaerophilic Fe(II) oxidation, anaerobic nitrate-dependent Fe(II) oxidation, and…
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Impact of dCO2 on Process Parameters: Effect of High dCO2 on Viable Cell Density and Product Titer in CHO Cell Culture
Real-time monitoring of bioprocess parameters is important to ensure reproducible cell culture and optimize product quality. Fast detection of unfavorable conditions in cell…
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Non-invasive Online CO2 Measurement with Chemical Optical CO2 Sensors: Chemical optical CO2 sensors provide a versatile tool for online monitoring of dissolved CO2 in various scale and designs
Accurate CO2 measurement in the liquid phase has been a challenge for decades. Although off-gas measurement by means of IR-spectroscopy is a proven technology, the correlation of…
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Advanced Respirometry with Chemical Optical Sensor Spots: Non-invasive Tracking of Oxygen, Carbon Dioxide and pH as Indicator for Nitrification Activity
Respirometry is an indirect way of measuring microbial activity, such as nitrification. The oxygen uptake is proportional to the substrate consumption - given by the stoichiometry…
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Ischemia-related Cell Damage in Extracorporeal Preserved Tissue: Assessing new findings with a novel perfusion model using optical pO2, pCO2 and pH sensors
Tissue undergoing free transfer in transplant or reconstructive surgery always is at high risk of ischemia-related cell damage. This study aims at assessing different procedures…
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Online pH Measurements in iTube pH Bioreactors: Evaluating the ITR iTube96Reader for Monitoring Insect Cell Culture Growth for Biopesticide Production
In our experiments we tested TubeSpin50 bioreactors with integrated pH sensors (referred to as iTube pH bioreactors). We evaluated their suitability for growing Lymantria dispar…
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