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Trace Oxygen Measurements in Perfusion Systems
Autoclavable O2 Flow-Through Cell FTC-PSt6-YAU
The FTC-PSt6-YAU is a miniaturized trace oxygen sensor integrated in a flow-through cell. A glass tube with an inner diameter of 2 mm is coated with the sensor at its inner wall. The FTC is connected to the oxygen meter with an optical fiber. The volume for liquid inside the FTC is about 100 microliter. The standard flow-through cell can be easily connected via Luer-Lock adapters to external tubings.
- Online oxygen measurements in perfusion systems
- No consumption of oxygen
- Signal independent of flow velocity
In-line Oxygen Monitoring in 3D Cell Culture
Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. PreSens flow-through cells with integrated chemical optical sensors can be incorporated in the construct inlet and outlet and allow in-line measurement of oxygen tension.
Application Note by R. Santoro, et al.: Perfusion Culture of Cell Seeded 3D Scaffolds for Tissue Engineering
|Specifications||Gaseous & Dissolved O2||Dissolved O2|
|*after two-point calibration as described in the manual|
|Measurement range|| |
0 – 5 % O20 – 41.4 hPa
0 – 2 mg/L0 – 56.9 µmol/L
|Limit of detection||0.002 % oxygen|
± 0.0007 % O2 at 0.002 % O2
± 0.0003 mg/L at 0.001 mg/L
|Accuracy*||± 1 ppb or ± 3 % of the respective concentration; whichever is higher|
|Drift||< 2 ppb within 30 days (sampling interval of 1 min./ at 0 % oxygen)|
|Measurement temperature range||from 0 to + 50 °C|
|Response time (t90)||< 6 sec.||< 40 sec.|
|Compatibility||Aqueous solutions, ethanol, methanol|
pH 1 – 14
|Cross-sensitivity||Organic solvents, such as acetone, toluene, chloroform or methylene chloride|
3 % H2O2
|Calibration||Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 1 % and 2 % oxygen|
|Storage stability||24 months provided the sensor material is stored in the dark|
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