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24 Deep Well Plate with Integrated pH Sensors for Read-out with the SDR
Deep Well HydroDish® HD24-DW
The Deep Well HydroDish® HD24-DW is a disposable polystyrene multidish and used for shaken cultures. Pre-calibrated pH sensors are integrated at the flat bottom of each square well and are read out with the SDR SensorDish® Reader. They are delivered beta-irradiated.
- For shaken cultures
- Manual calibration possible
- For microbes & cell cultures
Assessing Optimal Growth Conditions for Shaken Yeast Culture
The yeast P. guillermondii was cultivated in deep well Oxo- and HydroDishes to examine growth profiles and determine the minimal filling volumes for sensor read-out at a shaking speed of 200 rpm. As no optical shielding mask (SDR-OSM24) was used in these experiments, recordings show a higher noise-ratio at lower filling volumes. Both oxygen and pH kinetics show clearly when the initial substrate is consumed and the cultures switch to metabolise another substrate. This substrate change correlates with the filling volume, as low volume samples had better oxygen supply and grew faster, so the initial substrate was used up earlier. The optimal working volume for yeast cultivation in the deep well plates is 1.25 - 1.5 mL.
Thomas Grimm, BIOWORX, Berlin, Germany
|* in physiological solutions, 37 °C|
|Measurement range||pH 6.0 - 8.5|
|Resolution*||at pH = 7: ± 0.05 pH|
|Precision*||± 0.2 pH at pH = 7 (sensor batch calibration)|
± 0.1 pH at pH = 7 (sensor spot calibration)
|Drift*||< 0.1 pH within one week (sampling interval 10 min.)|
|Measurement temperature range||from + 15 to + 45 °C|
|Response time (t90)||at 25 °C: < 120 sec.|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10|
|Cross-sensitivity||Reduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere|
Disposables are delivered beta-irradiated
|Maximum filling volume||10 mL|
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