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24 Deep Well Plate with Integrated Oxygen Sensors for Read-Out with the SDR
Deep Well OxoDish® OD24-DW
The Deep Well OxoDish® OD24-DW is a disposable polystyrene multidish and used for shaken applications. Pre-calibrated oxygen sensors are integrated at the flat bottom of each square well and are read out with the SDR SensorDish® Reader. They are delivered beta-irradiated.
- For shaken cultures
- Manual calibration possible
- For microbes & cell cultures
Assessing Optimal Growth Conditions for Shaken Yeast Culture
The yeast P. guillermondii was cultivated in deep well Oxo- and HydroDishes to examine growth profiles and determine the minimal filling volumes for sensor read-out at a shaking speed of 200 rpm. As no optical shielding mask (SDR-OSM24) was used in these experiments, recordings show a higher noise-ratio at lower filling volumes. Both oxygen and pH kinetics show clearly when the initial substrate is consumed and the cultures switch to metabolise another substrate. This substrate change correlates with the filling volume, as low volume samples had better oxygen supply and grew faster, so the initial substrate was used up earlier. The optimal working volume for yeast cultivation in the deep well plates is 1.25 - 1.5 mL.
Thomas Grimm, BIOWORX, Berlin, Germany
Evaluating the SDR & OxoDishes® for Strain Development
Oxygen kinetics during a shaken E. coli cultivation were monitored using a Deep Well OxoDish®. Different media compositions were tested and compared to a minimal medium (MM). A higher concentration of yeast extract (YE) led to faster growth. Addition of glycerine as a second substrate prolonged the stationary phase. Ammonium chloride had no influence on the metabolism. After the substrate(s) was consumed, oxygen increased due to oxygen ingress.
Olaf Christensen, Lonza Ltd., Visp, Switzerland
|* in H2O dest. or oxygen-free water|
|Measurement range||0 - 50 % O2|
|Resolution*||± 0.4 % O2|
|Precision*||± 1 % O2|
|Drift*||< 0.2 % O2 within one week (sampling interval 10 min.)|
|Measurement temperature range||from + 15 °C to + 45 °C|
|Response time* (t90)||at + 25 °C: < 30 sec.|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10|
Disposables are delivered beta-irradiated
|Maximum filling volume||10 mL|
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