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Non-invasive pH Measurements in 6-Well Dishes
Pre-calibrated pH sensors are integrated at the bottom of each round well of this 6-well multidish and are read out with the SDR SensorDish® Reader non-invasively. The HD6 are delivered beta-irradiated and can be put inside an incubator.
- Manual calibration possible
- Ideal for cell cultivation & tissue engineering
|* in physiological solutions, 37 °C|
|Measurement range||pH 6.0 - 8.5|
|Resolution*||at pH = 7: ± 0.05 pH|
|Precision*||± 0.2 pH at pH = 7 (sensor batch calibration)|
± 0.1 pH at pH = 7 (sensor spot calibration)
|Drift*||< 0.1 pH within one week (sampling interval 10 min.)|
|Measurement temperature range||from + 15 to + 45 °C|
|Response time (t90)||at 25 °C: < 120 sec.|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10|
|Cross-sensitivity||Reduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere|
Disposables are delivered beta-irradiated
|Maximum filling volume||15 mL|
Can I re-use the SensorDishes®? Can I use the SDR with other formats than the 24-well plates? Do cells grow on the sensor inside the SensorDishes®? Do SensorDishes® come sterile? How do I import data into excel from a text file containing measurement data? How long can I use a SensorDish®? How long do I need to equilibrate before starting a SDR measurement? The SDR software shows "no sensor" instead of a value. What is the reason? What is the difference between User-Defined Calibration and One-Point Adjustment for the SDR? When do I apply which one? What is the time of delivery? Which substances can interfere with the optical O2, pH and CO2 measurements? Which toxicity tests where done with the SensorDish®? Why are there two different calibration data sets for SensorDishes®? Why do I need to equilibrate my samples for such long time periods before measurement with the SDR? Why do I see peaks in the graphs of the SDR software (especially in the oxygen signal) when I open the incubator door / take the plates out of the incubator? Why does the SDR software show pH<5, although I know that my sample is basic?