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Trace Oxygen Measurements with High Spatial Resolution

Needle-Type Oxygen Microsensor NTH-PSt8

The end of a glass fiber is coated with oxygen-sensitive material with a measurement range of 0 – 10 % oxygen. This tip (230 µm) is protected inside a stainless steel needle and can be extended for measurements. As long as the sensor tip is retracted and protected by the needle the microsensor can be pierced through septum rubber or any other harsh material. With its small tip size and fast response time (t90 < 3 s) this sensor is ideally suited for trace oxygen measurements in research and packaging applications.

  • High spatial resolution
  • High temporal resolution (t90 < 3 sec.)
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures liquids as well as in gas phase

Applications

Tissue Engineering

Oxygen microsensors measure the oxygen content in various volume compartments of the tissue engineering constructs. To do so, hair-thin sensors are inserted into the constructs and the oxygen content is measured online. In this way, the oxygen partial pressure is measured with a high local resolution and correlated with the constructs tissue quality (composition of the extracellular matrix).


Packaging & Quality Control

Oxygen inside packaging can lead to oxidative deterioration of certain products. Therefore, determination of the oxygen content within packages or pharmaceutical vials is of essential importance to ensure both the filling quality and the long-term storage stability. With our micro-invasive needle-type oxygen microsensors we offer a simple tool to determine residual oxygen both in the headspace and in liquids. The septum of the vial or package is pierced with the needle and the sensor is extended for measurement. As the measurement is made inside the package no error-prone and time consuming sampling is necessary. These oxygen microsensors are ideal tools for quality control in the food & beverage, packaging, and pharmaceutical industries.


Technical

Specifications*Gaseous & Dissolved O2Dissolved O2
*data for microsensors TF = flat-broken sensor tip with 230 μm diameter
**after two-point calibration as described in the manual
Measurement range0 – 10 % O2
0 – 100 hPa

0 – 4.5 mg/L

0 – 140 µmol/L
Limit of detection0.007 % oxygen3 ppb
Resolution

± 0.002 % O2 at 0.008 % O2
± 0.06 % O2 at 2.5 % O2

± 0.7 ppb at 3 ppb
± 2.5 ppb at 1000 ppb

Accuracy at + 20 °C**± 3 ppb or ± 3 % rel.
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 3 sec. (gas)< 10 sec. (liquid)
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity

Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas

Sterilization procedureEthylene oxide (EtO)
Cleaning procedure

3 % H2O2
Ethanol
Soap solution

CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 2 % and 4 % oxygen
Storage stability60 months provided the sensor material is stored in the dark at room temperature

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