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Measuring pH with High Spatial Resolution

Needle-type pH Microsensor NTH-HP5

The NTH-HP5 is designed for measuring in small volumes. The sensor is based on a 140 μm silica fiber which enables integration into manifold small scale environments. With its protective syringe needle housing the NTH-HP5 can easily penetrate tissue, septum rubber or packaging materials. After penetration the sensor tip is extended for measurements.

  • High spatial resolution
  • Measurement in smallest volumes
  • Profiling of pH gradients
  • Integration into plant and animal tissue
  • Optimized for culture media and physiological solutions

Applications

pH Measurement in Plants & Animals

pH Microsensors can be implemented in plant tissue or even in small animals. New insights in physiological aspects can be obtained. Due to the small size of the probes only a minimal disturbance will occur.


pH Measurement in Small Volumes

Due to the small dimension of the probe, pH measurements can be done in very small volumes - even in microtiter plates of a higher format like 384 or 1536. No need for reference electrodes - a real step forward, especially in small volumes. Of course, the measurement is independent of electromagnetical fields - this even allows measuring in NMR spectrometers.


Technical

Specifications*
* provided pH sensors are used without further handling in physiological solutions
Measurement range5.5 - 8.5 pH
Resolutionat pH = 7: ± 0.02 pH
Accuracyat pH = 7: ± 0.1 pH with sensor calibration
Driftat pH = 7: < 0.05 pH per day (sampling interval of 1 min.)
Measurement temperature rangefrom + 5 to + 50 °C
Response time (t90)at 25 °C: < 30 sec.
Properties*
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2- 10
No cross-sensitivityElectrical fields, proteins
Cross-sensitivityReduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
Sterilization procedureEthylene oxide (Et0), recalibration recommended
Cleaning procedureWater, Acrylan, pepsin solution
CalibrationpH sensors are pre-calibrated; recalibration is possible
Storage stability24 months provided the sensor is stored in the dark

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