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Disposable 24-Well Plate for Online Oxygen Monitoring

OxoDish® OD24

The OxoDish® OD24 is a disposable polystyrene low-well multidish. Pre-calibrated oxygen sensors are integrated at the bottom of each well and are read out contactless through the transparent well bottom with the SDR SensorDish® Reader. The OxoDishes® are delivered beta-irradiated.

  • Parallel online oxygen measurements
  • Ready-to-use
  • Pre-calibrated
  • Manual calibration possible
  • For microbial, tissue & cell cultures

Applications

More Security at Hypoxic Stem Cell Cultivation

The influence of medium change on dissolved oxygen (DO) at cultivation of human embryonic stem cells (hESC) was investigated at different oxygen tensions in the incubator atmosphere. Samples with full medium change using non-precalibrated medium showed a DO increase of 20 - 60 % air saturation. Other than expected, even half medium change with pre-incubated medium resulted in a notable DO increase of 10 - 30 % air saturation. The SensorDish® Reader can be used in hypoxia incubators, but also in small hypoxia chambers (see picture).

Barbara Ley, Prof. Oliver Brüstle, Life & Brain GmbH, Bonn, Germany


Oxygen and pH Monitoring in Tissue Engineering

Human chondrocytes with different start cell concentrations were cultivated in OxoDishes® and HydroDishes®. pH deviations from the control wells (medium only) could be detected even for the lowest start concentration. The acidification rates were in accordance with the different start concentrations. Medium change after 5 days led to a temporal pH increase until the samples were in equilibrium with the incubator atmosphere (5% CO2) again. Oxygen kinetics show the respective oxygen decrease.

Dr. Andreas Thomsen, CellGenix GmbH, Freiburg, Germany


High Throughput Screening of Water Oxidation Reactions with OxoDishes®

Splitting of water in a light-driven reaction is one of the most promising processes for producing clean fuel. In order to create an efficient process suitable reaction catalysts must be found. A high throughput screening of more than 200 reactions with different catalysts was performed using OxoDishes® and oxygen production was recorded. The example shows oxygen monitoring of complex 1 at different concentrations under different reaction conditions. The OxoDishes® and the SDR proved very useful for high throughput analyses and several catalysts that could form oxygen at the used reaction conditions could be identified.

R. J. Detz1, Z. Abiri1,2, A. M. Kluwer2, J. N. H. Reek1, 1Van't Hoff Institute for Molecular Sciences, University of Amsterdam, NL, 2InCatT B. V., Amsterdam, NL


Technical

Specifications
* in H2O dest. or oxygen-free water

Measurement range0 - 50 % O2
Resolution*± 0.4 % O2
Precision*± 1 % O2
Drift*< 0.2 % O2 within one week (sampling interval 10 min.)
Measurement temperature rangefrom + 15 to + 45 °C
Response time* (t90) at + 25 °C: < 30 sec.
Properties
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Maximum filling volume3 mL
CalibrationPre-calibrated
SensorDishes® are delivered beta-irradiated

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Resources

Publications

Quality Assessment of Primary Human Keratinocyte CulturesEnergy Catabolism Measurements in Metastatic Macrophage M3 CellsIdentifying Water Oxidation Catalysts for Clean Fuel ProductionLocalized Oxygen Depletion by Transmigrating Neutrophils Influences Inflammatory ResolutionCorrecting for Oxygen Diffusion in Respiration MeasurementsNon-Invasive Online Oxygen MeasurementProcess Monitoring in Suspension-Adapted CHO Cell CulturesOnline Oxygen Monitoring SystemA Novel Online Monitoring System3D Cultures of Disc-ChondrocytesOnline Oxygen Monitoring in Cell CultureDesign of Experiment (DoE) for Fed-batch Process Development in Shaken CulturesHigh Throughput Respiration Assay for Cytotoxicity DeterminationNon-invasive Method for Monitoring Real-Time Oxygen during Hematopoietic Stem/Progenitor Cell (HSPC) CultureReal Time Respiration MeasurementsCharacterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter platesHigh troughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurementsPioglitazone modulates tumor cell metabolism and proliferation in multicellular tumor spheroidsLactic Acid and Acidification Inhibit TNF Secretion and Glycolysis of Human MonocytesMonitoring pH and dissolved oxygen in mammalian cell culture using optical sensorsAnalysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cellsChemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro modelHigh glucose concentrations attenuate hypoxia-inducible factor-1α expression and signaling in non-tumor cellsReduced oxygen stress promotes propagation of murine postnatal enteric neural progenitors in vitroAdaptation to oxygen deprivation in cultures of human pluripotent stem cells, endothelial progenitor cells, and umbilical vein endothelial cellsDifferent stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxiaOxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samplesUnforeseen decrease in dissolved oxygen levels affect tube formation kinetics in collagen gelsNon-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterialsRespirometry: Correcting for Diffusion and Validating the Use of Plastic Multiwell Plates with Integrated OptodesMeasuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in cultureA Fluorescence-Based Screening Protocol for the Identification of Water Oxidation Catlysts

Calibration Data

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