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Oxygen Monitoring in 96-well Microtiterplate

OxoPlate OP96U

The OP96U is a round bottom 96-well multiplate with an oxygen sensor integrated on the bottom of each well. The plate is made of polystyrene. The sensors can be read out in parallel from the bottom side using a commercially available fluorescence reader.

  • Ready-to-use
  • User-defined batch calibration
  • For use with conventional microplate fluorescence readers
  • High throughput screening

Applications

Enzyme Kinetics Measurement

The OxoPlate OP96U can be used to determine enzyme activities. In this example kinetics of glucose oxidase (GOD) with different activities (0.05, 0.02, 0.01 and 0.05 U/mL) is shown. The OxoPlate filled with glucose solution and catalase (to avoid destruction of glucose oxidase by formed hydrogen peroxide) was incubated in the reader before the enzyme was added. Praffine oil was put into each well to reduce oxygen ingress. The oxygen measurement results show clearly the different dynamic equilibria of oxygen consumption and oxygen ingress depending on the added enzymes activity.

S. Arain, 2006, Microrespirometry with Sensor-Equipped Microtiterplates, University of Regensburg, Regensburg, Germany


Technical

Specifications*
* in H2O dest. or oxygen-free water
Measurement range0 - 30 % O2
Resolution± 0.2 % O2 at 0.21 % O2
± 0.6 % O2 at 20.9 % O2
Accuracy± 0.4 % O2 at 0.21 % O2
± 1.0 % O2 at 20.9 % O2
Drift< 0.04 % O2 per hour (sampling interval 1 min.)
Measurement Temperature Rangefrom + 15 °C to + 45 °C
Response time (t90) at + 25 °C: < 30 sec.
Properties
CompatibilityAqueous solutions, ethanol (max . 10 % v/v), methanol (max. 10 % v/v), pH 2 – 10, 20 % (v/v) DSMO, DMF, acetone, THF, or dioxane in solution. (Calibration constants have to be determined using the respective solvent instead of water.)
Cross sensitivityGaseous sulfur dioxide (SO2) and gaseous chlorine (Cl2); Changes in ionic strength
CalibrationNeeds to be calibrated once per batch
Disposables are delivered beta-irradiated
Plate type96-well round bottom plates (Greiner Microlon® 600)
Maximum filling volume300 µL
Indicator filters540 nm / 650 nm
Reference filters540 nm / 590 nm

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