Top

Oxygen Sensor Foil for Imaging Sample Surfaces or Through Transparent Vessel Walls

Oxygen Sensor Foil SF-RPSu4

The SF-RPSu4 for measuring oxygen allows non-invasive mapping of metabolic activities as well as changes over time periods from seconds to months. The fluorescent sensor foil is attached on a living or dead sample surface or a transparent glass or disposable vessel. A sensor film on the foil translates the oxygen content into a light signal. The sensor foil is
available in different sizes and can easily be cut in any desired shape. Read-out is done contactless with the imaging Detector Unit DU01 or the VisiSens TD.

  • 2D read-out
  • Contactless, direct sensing or through transparent walls
  • Visualize spatial and temporal gradients
  • Numerous measurement points in one image

Applications

O2, pH and CO2 Mapping in Sediments

O2, pH, and CO2 are key factors for microbial activity and various geochemical processes in sediments. They highly vary locally, e.g. at interfaces or different depths. Spatial and temporal analyte dynamics over long time periods can be visualized. Various regions can be compared within one measurement. VisiSens enables non-invasive 2D-mapping over cross-sections or on sample surfaces. The portable device can be used in lab and field.


Spatial and Temporal Analyte Changes in Plants & Soil

O2, pH and CO2 play a crucial role in plant and soil processes, e. g. in photosynthesis, respiration, in rhizospheres or in microbiological processes. Metabolic processes can be monitored. This planar optical sensor technique allows non-invasive read-out through glass walls of rhizotrons. Studying metabolic activity of roots and determining the cultivation optimum is important for sustainable agriculture, e. g. for adjustment of water and fertilizer supply.


O2 or pH in Cell Culture and Engineered Tissue

Cellular metabolism critically depends on local O2 supply and pH values. Especially in 2D and 3D cell culture or engineered tissue, cells located in diffusion limited regions (e.g. in scaffolds or spheroids) can be subject to low oxygen levels and pH changes. Non-invasive, continuous 2D-mapping can be performed directly in the incubator under growth conditions. Furthermore, 2D analyte distributions in living samples can be visualized.


Non-invasive 2D Analyte Mapping in Microfluidics

VisiSens™ enables 2D visualization of important culture parameters inside microfluidic chips. You can continuously monitor in 2D, with high resolution at specific positions or over the whole chip surface in a non-contact readout mode. Detect metabolic hotspots, record time-series, and monitor hypoxia, cellular growth, or O2 supply inside the chip. You can gain new insights on metabolic activity and natural or artificially produced gradients.


Technical

Specifications#
*typical data which may stronly differ with adapting the imaging set-up to specific needs
**typical data of LOD of a defined ROI (> 6,000 pixels) over time at + 20 °C, excluded ambient light, FoV 8 cm x 6 cm, VisiSens DU01 strongly differes
***typical data of precision of a defined ROI (> 6,000 pixels) over time at + 20 °C, excluded ambient light, FoV 8 cm x 6 cm, VisiSens DU01 strongly differes
**** typical data of spatial standard deviation in defined ROI (> 6,000 pixels) at + 20 °C, excluded ambient light, FoV 8 cm x 6 cm, VisiSens DU01 strongly differes
#VisiSens™ is no approved medical device

Measurement range0 – 100 % air saturation(0 – 20.9 % O2)
Response time* (t90)Gas phase: < 8 sec.         Dissolved: < 30 sec.
Specifications using VisiSens TD read-out
Limit of detection**0.03 % air saturation
Precision (temporal)***± 0.02 % air saturation at 0 % air saturation
± 0.1 % air saturation at 100 % air saturation
Precision (spatial)****± 1.5 % air saturation at 0 % air saturation
± 3.0 % air saturation at 100 % air saturation
Properties
General sensor temperature working rangefrom + 5 to + 45 °C
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Size of sensor foilStandard 40 x 40 mm²
Min. 5 x 5 mm²

Related products

2D O2 Imaging VisiSens™ Detector Unit DU01

Detector Unit DU01

The Detector Unit DU01 is an imaging technology allowing...

More
Screens of VisiSens™ AnalytiCal 1 software to control the VisiSens™ A1 oxygen imaging system

VisiSens™ AnalytiCal 1 Software

This software allows controlling the image recording process...

More
Adapter Tubes for VisiSens™ Detector Units

VisiSens™ Adapter Tubes

Tubes in different sizes can be attached to the VisiSens™...

More
Modular O2, pH and CO2 mapping system VisiSens TD

VisiSens TD

VisiSens TD enables simultaneous 2D read-out of optical O2,...

More
Screens of VisiSens™ ScientifiCal software to control the VisiSens TD imaging system

VisiSens™ ScientifiCal Software

VisiSens TD includes a modular control and evaluation...

More

Resources

Publications

Cellular Oxygen Consumption in Microfluidic DevicesRole of Oak Wood Ellagitannins on the Oxygen Transfer Rate in Wine BarrelsBenthic Disturbance-Recovery Dynamics in a Changing Coastal OceanImmobilized Plant Cells - From DSMZ to the CustomerDiffusive / Dispersive Reaction Fronts in GroundwaterExchange of O2 and CO2 in the Capillary Fringe of a Porous MediumMonitoring Oxygen in a Mouse Model of Renovascular HypertensionMeasuring Respiration and Primary Production Dynamics in MacroalgaeAssessment of Oxygen Depletion and Biofilm Structure Grown in MBBR CarriersMeasuring Oxygen Gradients above a Respiratory Active Algal Cell LayerViability of Lithobiontic Microorganisms Inhabiting the Rocks of Atacama DesertOxygen Dynamics in the Capillary FringeMonitoring Skin Tissue Oxygenation in MiceRecording Spatial Patterns of Oxygen Consumption in Individual CoralsEffect of Non-Symbiotic Hemoglobin Expression on Oxygen Content in RootsEffect of Pseudomonas Infection on Oxygen Status of Arabidopsis LeavesIntegration of Oxygen Sensor Foils in Microfluidic ChipsO2, CO2 and pH Dynamics in the Capillary FringeImaging the Oxygen Consumption of Microbial CulturesMonitoring pO2 in Cell Culture to Improve Directed DifferentiationOxygen Dynamics around Buried Tar Balls in Florida Marine SandsAssessing Biocide Actions on Lichen with VisiSensPerfusion Monitoring in Microvascular FlapsExtracorporeal Membrane Oxygenator to Support Diseased LungsRedox regulation of EGFR steers migration of hypoxic mammary cells towards oxygenDynamics of oxygen and carbon dioxide in rhizospheres of Lobelia dortmanna - a planar optode study of belowground gas exchange between plants and sedimentOxygen transport in periodically ventilated polychaete burrows2D visualization captures the local heterogeneity of oxidative metabolism across soils from diverse land-useHigh Resolution Assessment of Spatio-Temporal Changes in O2 Concentration in Root-Pathogen InteractionOxygen-distribution within 3-D collagen I hydrogels for bone tissue engineeringA microfluidic oxygen sink to create a targeted cellular hypoxic microenvironment under ambient atmospheric conditionsPlant-Sediment Interactions in Salt Marshes - An Optode Imaging Study of O2, pH, and CO2 Gradients in the RhizosphereSpatial organization of bacterial populations in response to oxygen and carbon counter-gradients in pore networksLinking biofilm spatial structure to real-time microscopic oxygen decay imagingAnoxic microsites in upland soils dominantly controlled by clay contentAn experimental-numerical investigation on the effects of macroporous scaffold geometry on cell culture parametersWho really matters: Influence of German Bight key bioturbators on biogeochemical cycling and sediment turnoverOxygen Mapping: Probing a Novel Seeding Strategy for Bone Tissue EngineeringWurzelexsudation in der Wasserfluktuationszone des Drei-Schluchten-Reservoirs, VR China. Ein Ansatz für Einblicke in eine überflutungsgestresste Rhizosphäre am Beispiel von Salix variegata FranchEffects of light fractionation and different fluence rates on photodynamic therapy with 5-aminolaevulinic acid in vivoTranscutaneous pO2 imaging during tourniquet-induced forearm ischemia using planar optical oxygen sensorsDetermination of Oxygen Gradients in Engineered Tissue Using a Fluorescent SensorMulti-ion imaging using fluorescent sensors in a microtiterplate array formatFluorescence Detection and Diagnosis of Non-Melanoma Skin Cancer at an Early StageFluorescent Imaging of pH with Optical Sensors Using Time Domain Dual Lifetime ReferencingLuminescence Lifetime Imaging of Oxygen, pH, and Carbon Dioxide Distribution Using Optical SensorsLuminescence Lifetime Temperature Sensing Based on Sol-Gels and Poly(acrylonitrile)s Dyed with Ruthenium Metal-Ligand ComplexesSimultaneous Imaging of Cortical Partial Oxygen Pressure and Anatomic Structures Using a Transparent Optical Sensor FoilTranscutaneous pO2 measurement during tourniquet-induced venous occlusion using dynamic phosphorescence imagingA new semi-invasive method for two dimensional pO2 measurements of cortical structuresNon-invasive measurement of the superficial cortical oxygen partial pressureRadial oxygen gradients over rat cortex arteriolesPlanar Oxygen Sensors for Non Invasive Imaging in Experimental BiologyVisualisation of Cortical pO2 During an Epidural Mass Lesion in RodentsSimple, fast and reliable perfusion monitoring of microvascular flapsAn imaging method for oxygen distribution, respiration and photosynthesis at a microscopic level of resolutionCharacterization of a chip-based bioreactor for three-dimensional cell cultivation via magnetic resonance imagingRatiometric luminescence 2D in vivo imaging and monitoring of mouse skin oxygenationReal-Time 2D Visualization of Metabolic Activities in Zebrafish Embryos Using a Microfluidic TechnologyLow-oxygen tensions found in Salmonella-infected gut tissue boost Salmonella replication in macrophages by impairing antimicrobial activity and augmenting Salmonella virulenceOnline Monitoring of Crude Oil Biodegradation at Elevated PressuresEffect of Farnesyltransferase Inhibitor R115777 on Mitochondria of Plasmodium falciparumUltrasonic welding of chemical optical sensors supporting O2, pH and CO2 imaging in microfluidic systemsOxygen measurement in interstitially perfused cellularized constructs cultured in a miniaturized bioreactor

Presens TV

Watch tutorials, webinars and informative videos about PreSens optical sensor systems.

All videos