Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
Oxygen Sensor Foil for Imaging Sample Surfaces or Through Transparent Vessel Walls
Oxygen Sensor Foil SF-RPSu4
The SF-RPSu4 for measuring oxygen allows non-invasive mapping of metabolic activities as well as changes over time periods from seconds to months. The fluorescent sensor foil is attached on a living or dead sample surface or a transparent glass or disposable vessel. A sensor film on the foil translates the oxygen content into a light signal. The sensor foil is
available in different sizes and can easily be cut in any desired shape. Read-out is done contactless with the imaging Detector Unit DU01 or the VisiSens TD.
- 2D read-out
- Contactless, direct sensing or through transparent walls
- Visualize spatial and temporal gradients
- Numerous measurement points in one image
O2, pH and CO2 Mapping in Sediments
O2, pH, and CO2 are key factors for microbial activity and various geochemical processes in sediments. They highly vary locally, e.g. at interfaces or different depths. Spatial and temporal analyte dynamics over long time periods can be visualized. Various regions can be compared within one measurement. VisiSens enables non-invasive 2D-mapping over cross-sections or on sample surfaces. The portable device can be used in lab and field.
Spatial and Temporal Analyte Changes in Plants & Soil
O2, pH and CO2 play a crucial role in plant and soil processes, e. g. in photosynthesis, respiration, in rhizospheres or in microbiological processes. Metabolic processes can be monitored. This planar optical sensor technique allows non-invasive read-out through glass walls of rhizotrons. Studying metabolic activity of roots and determining the cultivation optimum is important for sustainable agriculture, e. g. for adjustment of water and fertilizer supply.
O2 or pH in Cell Culture and Engineered Tissue
Cellular metabolism critically depends on local O2 supply and pH values. Especially in 2D and 3D cell culture or engineered tissue, cells located in diffusion limited regions (e.g. in scaffolds or spheroids) can be subject to low oxygen levels and pH changes. Non-invasive, continuous 2D-mapping can be performed directly in the incubator under growth conditions. Furthermore, 2D analyte distributions in living samples can be visualized.
Non-invasive 2D Analyte Mapping in Microfluidics
VisiSens™ enables 2D visualization of important culture parameters inside microfluidic chips. You can continuously monitor in 2D, with high resolution at specific positions or over the whole chip surface in a non-contact readout mode. Detect metabolic hotspots, record time-series, and monitor hypoxia, cellular growth, or O2 supply inside the chip. You can gain new insights on metabolic activity and natural or artificially produced gradients.
|*typical data which may stronly differ with adapting the imaging set-up to specific needs|
**typical data of LOD of a defined ROI (> 6,000 pixels) over time at + 20 °C, excluded ambient light, FoV 8 cm x 6 cm, VisiSens DU01 strongly differes
***typical data of precision of a defined ROI (> 6,000 pixels) over time at + 20 °C, excluded ambient light, FoV 8 cm x 6 cm, VisiSens DU01 strongly differes
**** typical data of spatial standard deviation in defined ROI (> 6,000 pixels) at + 20 °C, excluded ambient light, FoV 8 cm x 6 cm, VisiSens DU01 strongly differes
#VisiSens™ is no approved medical device
|Measurement range||0 – 100 % air saturation(0 – 20.9 % O2)|
|Response time* (t90)||Gas phase: < 8 sec. Dissolved: < 30 sec.|
|Specifications using VisiSens TD read-out|
|Limit of detection**||0.03 % air saturation|
|Precision (temporal)***||± 0.02 % air saturation at 0 % air saturation|
± 0.1 % air saturation at 100 % air saturation
|Precision (spatial)****||± 1.5 % air saturation at 0 % air saturation|
± 3.0 % air saturation at 100 % air saturation
|General sensor temperature working range||from + 5 to + 45 °C|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10|
|Size of sensor foil||Standard 40 x 40 mm²|
Min. 5 x 5 mm²
Detector Unit DU01
The Detector Unit DU01 is an imaging technology allowing...
VisiSens™ AnalytiCal 1 Software
This software allows controlling the image recording process...
VisiSens™ Adapter Tubes
Tubes in different sizes can be attached to the VisiSens™...
VisiSens TD enables simultaneous 2D read-out of optical O2,...
VisiSens™ ScientifiCal Software
VisiSens TD includes a modular control and evaluation...
Video: VisiSens - Oxygen Distributions in E. coli Culture
Video: VisiSens - Visualizing Biocide Actions on Lichen
Video: VisiSens - Oxygen Imaging in Microfluidics
Video: VA1 Basic Functions
Video: VA1 Measurement
Video: VA1 Calibration
Video: VisiSens Delivered Equipment
Video: How2 Measure and Visualize O2, pH and CO2 in 2D for Biological Research
Video: How2 Measure and Visualize O2, pH and CO2 in 2D for Life Science Research
Video: VisiSens WEBINAR - Metabolic Activity inside Microfluidics
Video: VisiSens WEBINAR - O2, pH & CO2 in Sediments, Interfaces and Biofilms
Video: VisiSens WEBINAR - O2, pH & CO2 in Plants, Roots and Soil
Video: VisiSens WEBINAR - O2 & pH in Cell Culture, Engineered & Native Tissue
Media Release: Oxygen in Action
Media Release: Microfluidic Devices