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Trace Oxygen Measurements under Real Conditions

Oxygen Sensor Spot SP-PSt6-NAU

Sensor spots are the most versatile version of non-invasive optical oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. used for packaging. Oxygen is measured contactless through the transparent vessel wall. The SP-PSt6-NAU has a measurement range of 0 – 5 % oxygen, dissolved or in the gas phase. The oxygen sensitive coating is immobilized on a 125 µm flexible transparent polyester foil, which does not stand autoclaving.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase

Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 5  % O2

0 – 41.4 hPa

0 – 2 mg/L

0 – 56.9 µmol/L
Limit of detection0.002 % oxygen

1 ppb

Resolution

± 0.0007 % O2 at 0.002 % O2
± 0.0015 % O2 at 0.2 % O2
± 0.007 hPa at 0.023 hPa

± 0.015 hPa at 2.0 hPa

± 0.0003 mg/L at 0.001 mg/L
± 0.0006 mg/L at 0.09 mg/L
± 0.010 µmol/L at  0.03µmol/L

± 0.020 µmol/L at 2.8 µmol/L
Accuracy*± 1 ppb or ± 3 % of the respective concentration; whichever is higher
Drift at 0 % oxygen< 2 ppb within 30 days (sampling interval of 1 min.)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure

Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 % – 5 %

CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 1 % and 2 % oxygen
Storage stability24 months provided the sensor material is stored in the dark

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