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Trace Oxygen Measurements under Real Conditions
Oxygen Sensor Spot SP-PSt6-NAU
Sensor spots are the most versatile version of non-invasive optical oxygen sensors. They can be attached to the inner surface of any transparent glass or plastic vessel like e. g. used for packaging. Oxygen is measured contactless through the transparent vessel wall. The SP-PSt6-NAU has a measurement range of 0 – 5 % oxygen, dissolved or in the gas phase. The oxygen sensitive coating is immobilized on a 125 µm flexible transparent polyester foil, which does not stand autoclaving.
- Non-invasive measurements through the vessel wall
- No consumption of oxygen
- Signal independent of flow velocity
- Measures oxygen in liquids as well as in gas phase
Bioprocess Development: Oxygen Monitoring in Shake Flasks
O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.
Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials
Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.
|Specifications||Gaseous & Dissolved O2||Dissolved O2|
|*after two-point calibration as described in the manual|
|Measurement range|| |
0 – 5 % O20 – 41.4 hPa
0 – 2 mg/L0 – 56.9 µmol/L
|Limit of detection||0.002 % oxygen|
± 0.0007 % O2 at 0.002 % O2
± 0.0003 mg/L at 0.001 mg/L
|Accuracy*||± 1 ppb or ± 3 % of the respective concentration; whichever is higher|
|Drift at 0 % oxygen||< 2 ppb within 30 days (sampling interval of 1 min.)|
|Measurement temperature range||from 0 to + 50 °C|
|Response time (t90)||< 6 sec.||< 40 sec.|
|Compatibility||Aqueous solutions, ethanol, methanol|
pH 1 – 14
|Cross-sensitivity||Organic solvents, such as acetone, toluene, chloroform or methylene chloride|
Ethylene oxide (EtO)
Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
|Calibration||Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 1 % and 2 % oxygen|
|Storage stability||24 months provided the sensor material is stored in the dark|
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