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Measurements Under Real Conditions with Pre-Calibrated Sensors
pH Sensor Spots SP-HP5
The SP-HP5 allow online monitoring and are optimized for physiological solutions and culture media. The spots are mounted in transparent vessels made of glass or plastic and then read out contactless from the outside - through the container wall. The sensors are pre-calibrated and ready-to-use.
- Online monitoring without sampling
- Contactless measurements through the vessel wall
- Applicable from microliter to production scale
- Optimized for culture media and physiological solutions
Bioprocess Development: pH Monitoring in Shake Flasks
The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used. The combination with non-invasive oxygen sensors in shake flasks provides new insights into metabolic activity and changes in metabolic pathways.
|* provided pH senosrs are used without further handling in physiological solutions|
** equilibrated sensor kept in well stirred solution at + 37 °C
|Measurement range||5.5 - 8.5 pH|
|Resolution||at pH = 7: ± 0.01 pH|
|Accuracy||at pH = 7: ± 0.05 sensor spot calibration|
at pH = 7: ± 0.10 sensor batch calibration
|Drift||at pH = 7: < 0.005 pH per day (sampling interval of 1 min.)|
|Measurement temperature range||from + 5 to + 50 °C|
|Response time (t90)**||< 120 sec.|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10|
|Cross-sensitivty||Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere|
|Cleaning procedure||pH spots are delivered either beta-irradiated or untreated, a second irradiation or ethylene oxide treatment is not recommended|
|Calibration||pH spots are pre-calibrated; re-calibration is possible|
|Storage stability||18 months provided the sensor is stored in the dark|
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