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High Resolution Oxygen Profiling in Semi-Solid Samples

Profiling Oxygen Microsensor PM-PSt7

The PM-PSt7 is one of the most robust oxygen microsensor PreSens offers – with a firmer fiber construction and a splash-proof metal housing. It is specifically designed for profiling applications and should be used whenever minimal invasive measurements need to be performed in semi-solid samples, e. g. in sediments, microbial mats or biofilms. The PM-PSt7 is compatible with the Microx 4 and OXY-1 / -4 ST series oxygen meters and has a measurement range of 0 – 100 % oxygen, with a detection limit of 0.03 % oxygen. Together with the Automated Micromanipulator and the software PreSens Profiling Studio boundary layers and gradients can be followed online, while the sensor is automatically moved inside the sample.

  • High spatial resolution (< 50 μm)
  • High temporal resolution (t90 < 3 sec.)
  • Penetration probe for insertion into semi-solids
  • Mechanical interlock for precise vertical localization inside the sample
  • Splash-proof metal housing
  • No electrical interference due to optical measurements
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Applications

Microprofiling in Biological & Environmental Research

The different types of oxygen and pH microsensors allow e. g. measurements in smallest sample volumes or inside tissue. The micromanipulators should be applied whenever it is necessary to insert the microsensor safely into semi-solid samples and when exact localization and stabilization of the microsensor tip within the sample is required. Using the safe-insert function the microsensor tip can be securely inserted and localized at the exact position where you want to conduct your measurements.


Microsensor Measurements in Medical & Life Science Research

PreSens microsensors are ideal tools for medical and life science research, as they allow for precise on the spot measurement and microprofiling inside tissue constructs. The Manual Micromanipulator is the indispensable equipment in these applications for exact localization of the microsensor inside the sample and microprofiling in step sizes down to 10 μm. PreSens needle-type microsensors are used in many tissue engineering applications.


Microprofiling of Sediments & Biofilms

Together with the specially designed PreSens Profiling Microsensors (PM) the Automated Micromanipulator is the ideal tool for oxygen measurements in sediment and biofilm applications. With a free choice of step zones, travel velocities and wait times different layers inside the sample can be monitored and assessed in step sizes down to 10 μm. The software visualizes the online measurements, so you can follow gradients and identify boundaries immediately while the sensor is automatically moved inside the sample.


Technical

Specifications* Gaseous & Dissolved O2 Dissolved O2
*data for microsensor TF = flat-broken sensor tip with 230 μm diameter
**after two-point calibration as described in the manual
Measurement range 0 – 100 % O2
0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection 0.03 % oxygen 15 ppb
Resolution

± 0.01 % O2 at 1  % O2
± 0.05 % O2 at 20.9 % O2

± 0.005 mg/L at  0.4mg/L

± 0.025 mg/L at 9.06 mg/L
Accuracy at + 20 °C** ± 0.05 % O2 or ± 3 % rel.
Measurement temperature range from 0 to + 50 °C
Response time (t90) < 3 sec. (gas) < 10 sec. (liquid)
Properties    
Compatibility Aqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity

Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas

Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure

3 % H2O2
Ethanol
Soap solution

Calibration Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability 60 months provided the sensor material is stored in the dark at room temperature

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