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Robust Microsensor for pH Profiling in Semi-solids
Profiling pH Microsensor PM-HP5
The pH Profiling Microsensor can be used for all profiling applications in semi-solid substrates, such as sediments, microbial mats or biofilms. The PM-HP5 is the most robust version of PreSens pH Microsensors and has a close-fitting fiber guidance and mechanical interlock for precise vertical localization of the measurement tip. The sensor tip is extendable with a turning mechanism.
- Close-fitting fiber guidance
- Mechanical interlock
- High spatial resolution
- Measurement in smallest volumes
- Profiling of pH gradients
Applications
Microprofiling in Biological & Environmental Research
The different types of oxygen and pH microsensors allow e. g. measurements in smallest sample volumes or inside tissue. The micromanipulators should be applied whenever it is necessary to insert the microsensor safely into semi-solid samples and when exact localization and stabilization of the microsensor tip within the sample is required. Using the safe-insert function the microsensor tip can be securely inserted and localized at the exact position where you want to conduct your measurements.
Microsensor Measurements in Medical & Life Science Research
PreSens microsensors are ideal tools for medical and life science research, as they allow for precise on the spot measurement and microprofiling inside tissue constructs. The Manual Micromanipulator is the indispensable equipment in these applications for exact localization of the microsensor inside the sample and microprofiling in step sizes down to 10 μm. PreSens needle-type microsensors are used in many tissue engineering applications.
Microprofiling of Sediments & Biofilms
Together with the specially designed PreSens Profiling Microsensors (PM) the Automated Micromanipulator is the ideal tool for oxygen measurements in sediment and biofilm applications. With a free choice of step zones, travel velocities and wait times different layers inside the sample can be monitored and assessed in step sizes down to 10 μm. The software visualizes the online measurements, so you can follow gradients and identify boundaries immediately while the sensor is automatically moved inside the sample.
Technical
| Specifications* | |
|---|---|
| *provided pH sensors are used without further handling in physiological solutions **calibration and following measurements in the same conditions / system; equilibrated sensor kept in well stirred solution at + 37 °C | |
| Measurement range | 5.5 - 8.5 pH |
| Resolution | at pH = 7: ± 0.02 pH |
| Accuracy** | at pH = 7: ± 0.1 pH with sensor calibration |
| Drift | at pH = 7: < 0.05 pH per day (sampling interval of 1 min.) |
| Measurement temperature range | from + 5 to + 50 °C |
| Response time (t90)** | at 25 °C: < 30 sec. |
| Properties* | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| No cross-sensitivity | Electrical fields, proteins |
| Cross-sensitivity | Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere |
| Sterilization procedure | Ethylene oxide (Et0), recalibration recommended |
| Cleaning procedure | Water, Acrylan, pepsin solution |
| Calibration | pH sensors are pre-calibrated, recalibration is possible |
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FAQs
Can I change the cannula on my Profiling Microsensor?
Can I measure inside a tissue with fiber optic microsensors?
Can the Profiling Microsensor be submerged in water?
How can I prevent oxygen or pH microsensors from breaking when measuring profiles across tissue?
How do I import data into excel from a text file containing measurement data?
How does temperature affect my pH measurement?
How to prepare buffers of constant ionic strength and calibrate optical pH sensors?
The needle tip of the microsensor is vibrating or not moving smoothly when I try to insert it in the sample with the Manual Micromanipulator. What can I do?
What cannula / steel needle sizes are available for Profiling Microsensors?
What is the correct way for handling an oxygen or pH microsensor?
What is the difference between tapered and flat-broken microsensor tips?
What is the smallest tip size available for Profiling Microsensors?
What is the time of delivery?
Which buffers can be used for calibration of chemical optical pH sensors?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Why does my pH sensor not work in tap water?