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Non-invasive Online Culture Monitoring in Multiwell Plates

SDR SensorDish® Reader Basic Set

The SDR SensorDish® Reader is a small 24-channel reader for non-invasive detection of oxygen and pH in multidishes (SensorDishes®). These contain a sensor spot at the bottom of each well. They are read out non-invasively through the transparent bottom. SensorDishes® for oxygen (OxoDish®) and pH (HydroDish®) are available in 24-well and 6-well format. 24-well deep well plates with integrated oxygen (OxoDish®-DW) and pH sensor (HydroDish®-DW) allow measurements in shaken cultures. Read out of oxygen sensors integrated in glass vessels for respiration monitoring is also possible. The SensorDish® Reader can be used in incubators and on shakers and is thus the ideal tool for cell and bacteria cultivation.

  • Parallel online monitoring in disposable 24- or 6-well plates
  • Non-invasive & non-destructive measurement
  • Deep well plates (24-well format) & low well plates available
  • Pre-calibrated
  • For use in incubators and on shakers
  • Optional extension for monitoring of up to 240 samples

Applications

More Security at Hypoxic Stem Cell Cultivation

The influence of medium change on dissolved oxygen (DO) at cultivation of human embryonic stem cells (hESC) was investigated at different oxygen tensions in the incubator atmosphere. Samples with full medium change using non-precalibrated medium showed a DO increase of 20 - 60 % air saturation. Other than expected, even half medium change with pre-incubated medium resulted in a notable DO increase of 10 - 30 % air saturation. The SensorDish® Reader can be used in hypoxia incubators, but also in small hypoxia chambers (see picture).

Barbara Ley, Prof. Oliver Brüstle, Life & Brain GmbH, Bonn, Germany


Oxygen and pH Monitoring in Tissue Engineering

Human chondrocytes with different start cell concentrations were cultivated in OxoDishes® and HydroDishes®. pH deviations from the control wells (medium only) could be detected even for the lowest start concentration. The acidification rates were in accordance with the different start concentrations. Medium change after 5 days led to a temporal pH increase until the samples were in equilibrium with the incubator atmosphere (5% CO2) again. Oxygen kinetics show the respective oxygen decrease.

Dr. Andreas Thomsen, CellGenix GmbH, Freiburg, Germany


Evaluating the SDR & OxoDishes® for Strain Development

Oxygen kinetics during a shaken E. coli cultivation were monitored using a Deep Well OxoDish®. Different media compositions were tested and compared to a minimal medium (MM). A higher concentration of yeast extract (YE) led to faster growth. Addition of glycerine as a second substrate prolonged the stationary phase. Ammonium chloride had no influence on the metabolism. After the substrate(s) was consumed, oxygen increased due to oxygen ingress.

Olaf Christensen, Lonza Ltd., Visp, Switzerland


Real-time Monitoring of the Respiration of Marine Zooplankton

Oxygen consumption of 3 - 4 copepod nauplii per sample was monitored in air-tight glass vials for 6 h. The nauplii were offered phytoplankton at environmental concentrations. Feeding and faecal pellet production rates were estimated simultaneously. The respiration rates were linear and steady, thus revealing that the nauplii were neither influenced by the vessel walls nor by diminishing of food. The respiration rate was compared to literature values of other species.The higher oxygen consumption of the nauplii was presumably due to constant feeding.

Dr. Marion Köster, Ernst-Moritz-Arndt-Universität Greifswald, Germany, Mar Ecol Prog Ser 353: 157-164 (2008)


Technical

SpecificationspH*Oxygen
* In physiological solutions at 37 °C
Measuring range6.0 - 8.5 pH0 - 50 % O2
Resolution*± 0.05 pH at = 7± 0.4 % O2 at 20.9 % O2
Precision*± 0.2 pH at pH = 7 (sensor batch calibration)
± 0.1 pH at pH = 7 (sensor spot calibration)

± 1 % O2 at 20.9 % O2

Drift*< 0.1 pH within one week (sampling interval 10 min.)< 0.2 % O2 within one week (sampling interval 10  min.)
Measurement temperature rangefrom + 15 °C to + 45 °C
Response time (t90) at 25 °C< 30 sec.< 120 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol (max. 10 % v/v), pH 2 - 10
Cross-sensitivityReduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere
CalibrationHydroDishes® and OxoDishes® are pre-calibrated
DeviceSensorDish® ReaderSplitterPower adapter
TypeSDR v3 or higherSP1.1 or higherMascot 9920
CleaningEthanol
Input18 - 24 V DC 150 mA18 - 24 V DC 1.5 A100 - 240 V AC 50 - 60 Hz. max. 0.9 A
Weight380 g240 g
Dimensions16.3 cm x 8.9 cm x 2.2 cm12.4 cm x 8.0 cm x 4.5 cm

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Resources

Publications

Quality Assessment of Primary Human Keratinocyte CulturesEnergy Catabolism Measurements in Metastatic Macrophage M3 CellsIdentifying Water Oxidation Catalysts for Clean Fuel ProductionEvaluating the SDR SensorDish Reader® for Strain DevelopmentOxygen and pH Monitoring in Deep Well OxoDishes® and HydroDishes®Localized Oxygen Depletion by Transmigrating Neutrophils Influences Inflammatory ResolutionCorrecting for Oxygen Diffusion in Respiration MeasurementsNon-Invasive Online Oxygen MeasurementProcess Monitoring in Suspension-Adapted CHO Cell CulturesOnline Oxygen Monitoring SystemA Novel Online Monitoring System3D Cultures of Disc-ChondrocytesOnline Oxygen Monitoring in Cell CultureCo-Cultures of Caco-2 cells and E.coliDesign of Experiment (DoE) for Fed-batch Process Development in Shaken CulturesHigh Throughput Respiration Assay for Cytotoxicity DeterminationNon-invasive Method for Monitoring Real-Time Oxygen during Hematopoietic Stem/Progenitor Cell (HSPC) CultureReal Time Respiration MeasurementsCharacterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter platesHigh troughput, non-invasive and dynamic toxicity screening on adherent cells using respiratory measurementsOxygen consumption of doliolids (Tunicata, Thaliacea)Pioglitazone modulates tumor cell metabolism and proliferation in multicellular tumor spheroidsLactic Acid and Acidification Inhibit TNF Secretion and Glycolysis of Human MonocytesReal-Time screening system using living cells for chemosensitivity testingMonitoring pH and dissolved oxygen in mammalian cell culture using optical sensorsAnalysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cellsTime-series measurements of oxygen consumption of copepod naupliiChemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro modelInterference of magnesium corrosion with tetrazolium-based cytotoxicity assaysHigh glucose concentrations attenuate hypoxia-inducible factor-1α expression and signaling in non-tumor cellsReduced oxygen stress promotes propagation of murine postnatal enteric neural progenitors in vitroAdaptation to oxygen deprivation in cultures of human pluripotent stem cells, endothelial progenitor cells, and umbilical vein endothelial cellsDifferent stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxiaOxygen requirements of zebrafish (Danio rerio) embryos in embryo toxicity tests with environmental samplesUnforeseen decrease in dissolved oxygen levels affect tube formation kinetics in collagen gelsNon-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterialsOxygen consumption of fecal pellets of doliolids (Tunicata, Thaliacea) and planktonic copepods (Crustacea, Copepoda)Respirometry: Correcting for Diffusion and Validating the Use of Plastic Multiwell Plates with Integrated OptodesMeasuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in cultureA Fluorescence-Based Screening Protocol for the Identification of Water Oxidation Catlysts

FAQs

Are there some general recommendations for measurements with the SDR?Can I measure photosynthesis with the SDR?Can I save Trend / Arithmetic calculations done in the SDR software? Can I do them for all channels simultaneously?Can I use a different measurement interval for each connected SDR?Can I use the SDR for hypoxic measurements?Can I use the SDR for measurement of photosynthesis?Can I use the SDR with other formats than the 24-well plates?Can the same meter be used for pH, oxygen or CO2 sensors?How can I rename a measurement recorded with the SDR?How do I fix the SDR on a shaker?How do I import data into excel from a text file containing measurement data?How long do I need to equilibrate before starting a SDR measurement?I forgot to change the oxygen unit in the SDR software from the default unit "air saturation" to my preferred unit. How do I get my preferred oxygen unit?I have more than one SDR connected. Can I choose which SDRs I want to use for measurement?I use the OxoHydroDish (OHD6) with the SDR. How do I see the values of both parameters (oxygen and pH) with the SDR software?Is the sensor spot inside the SensorVials for read-out with the SDR the same as the ones read out with the other oxygen meters?Is the temperature which I see in the maintenance window of the SDR software used for temperature compensation?The SDR software says 'No SDR found'. What can I do?The SDR software shows "no sensor" instead of a value. What is the reason?What air pressure value should I enter in the SDR software? Where do I get it from?What happens if I cross the 30 °C-line during measurement with the SDR?What is salinity? What value should I enter in the SDR software?What is the difference between the 2 black masks for the SDR, the SDR-OSM24 and SDR-MSV24?What is the difference between User-Defined Calibration and One-Point Adjustment for the SDR? When do I apply which one?What is the time of delivery?Which measurement interval should I use for measurements with the SDR?Why are there two different calibration data sets for SensorDishes®?Why can't I see running and uploaded measurements simultaneously in the SDR software?Why can't I upload a previous measurement in the SDR software?Why do I need to equilibrate my samples for such long time periods before measurement with the SDR?Why do I see peaks in the graphs of the SDR software (especially in the oxygen signal) when I open the incubator door / take the plates out of the incubator?Why does the SDR software show pH<5, although I know that my sample is basic?

Software

SDR version v4.0.0 - Windows XP/Vista/7/8
USB Serial Driver - Windows XP/Vista
USB Serial Driver - Windows 7/8/8.1

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