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pH, Oxygen and OUR Monitoring in Shake Flasks, Cultivation Tubes and T-flasks
SFR Shake Flask Reader
The SFR Shake Flask Reader monitors pH, oxygen and OUR in up to 9 Erlenmeyer flasks, cultivation tubes, or T-flasks simultaneously. It fits in nearly all standard shakers. Measurement data is transferred wirelessly via Bluetooth to your PC / notebook. The corresponding ready-to-use vessels contain pre-calibrated sensor spots. The system monitors non-invasively through the transparent bottom of the container. Different types and sizes of flasks and tubes are available. Plastic, disposable flasks contain pre-calibrated oxygen and pH sensors, while re-usable glass flasks are equipped with autoclavable oxygen sensors.
- Fast monitoring of up to 63 shake flasks in parallel
- For microbial and cell culture
- Pre-calibrated cultivation vessels are ready-to-use
- Compatible with standard shakers
- Glass & plastic flasks in different sizes available
- Non-invasive measurement
- Used in seed train & bioprocess development
Process Monitoring in Suspension-Adapted CHO Cell Cultures
The online measurement of dissolved oxygen concentration and pH in shaken bioreactors paves the way for proper scale down activities from bench-top stirred-tanks to smaller scales. Adjustment of shaking speed as a function of pO2 is now possible avoiding possible oxygen limitations at high cell densities. Even a simple pH readjustment by tuning the pCO2 in the incubator is feasible to optimize the output from simple experiments with shaken bioreactors.
Dr. Robert Puskeiler, Roche Diagnostics, Penzberg, Germany
Yeast & E. coli: Ensure Enough Oxygen Supply
S. cerevisiae grows on different sugars as carbon source. While growth on glucose and fructose is mainly fermentative, growth on galactose is mainly respirative. This leads to low oxygen concentration in the shake flasks. The accurately measured oxygen indicates the need to increase rotation frequency to avoid oxygen limitation.
High oxygen demand is typical for E. coli in its exponential phase. In the cultivation shown on the left, rotation speed had to be changed twice in order to avoid oxygen limitation. In addition, changes in the metabolism can be detected by measuring DO.
Schneider et al., University of Saarland, Saarbrücken, GermanyBioprocess Biosyst Eng., 33(5), 541 - 547, 2009
* provided Sensor Flasks are used without further handling in physiological solutions
|Measurement range||0 – 100 % O2||5.5 – 8.0 pH|
|Response time (t90) at 25 °C||< 60 sec.||< 60 sec.|
± 0.01 % O2 at 0.21 % O2
|± 0.01 pH at pH = 7**|
± 0.4 % O2 at 20.9 % O2
± 0.1 pH at pH = 7 with one-point adjustment± 0.2 pH at pH = 7 with pre-calibration
|Drift||< 0.01 % O2 per day (sampling interval of 1 min.)||< 0.01 pH per day (sampling interval of 1 min.)|
|Temperature range||from + 5 °C to + 50 °C|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 4|
|Cross-sensitivity||Typically no cross-sensitivity in culture media||Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere|
|Sensor flasks are delivered irradiated|
Video: SFR Shake Flask Reader
Video: Shake Flask Clamps
Video: Glass Flask Clamps
Video: iTube Adapter
Media Release: Single-use Containers Demand Single-use Sensors
Media Release: Where does the Oxygen Go?
Media Release: Novel Single-use Sensors for Online Measurement of Glucose
Media Release: Enhancing Data Quality with a Partly Controllable System at Shake Flask Scale
Media Release: Shake Flask Feedback Controlled Feeding