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Contactless pH Measurements in Perfusion Systems

Single-Use pH Flow-Through Cell FTC-SU-HP5-US

The FTC-SU-HP5-US consists of a miniaturized optical pH sensor on a color coded stick, which is attached to a single-use T-cell. The sensor stick can be mounted on flow-through cells of different size and shape, according to requirements. The sensor inside the FTC is connected to the pH meter via polymer optical fiber and allows contactless measurements in perfusion systems. The FTC-SU-HP5-US is made of polycarbonate.

  • Single-use flow-through cell
  • Precise online monitoring of pH in perfusion systems
  • Different sizes and shapes for various flow rates
  • Easy connection to external tubing
  • Customized connectors are available, e. g. CPC

Applications

pH Monitoring in Liver Cell Bioreactor

An important aspect of efficient liver cell bioreactors is the automated regulation of physio-chemical culture parameters. A non-invasive pH regulation device for a perfusion bioreactor has been developed. The high performance of the system is based on one of our chemical optical flow-through cells for pH detection and its combination with precision mass-flow controllers for gas. The new controller allows long time stable and contamination free online pH regulation in complex bioreactor systems - an important technical contribution for future clinical applications.

Application Note by Marco Decker: pH Regulation for a Liver Cell Bioreactor


Online Measurement in Perfusion Systems

Beta-irradiated and pre-calibrated pH and DO flow-through sensors can be integrated into perfusion systems. This allows easy control of process parameters in perfusion reactors. Typically Luer connectors are used, though different sizes for larger or smaller flow rates are available.


Technical

Specifications*

* provided pH sensors are used without further handling in physiological solution
** after single point calibration at pH = 7
*** equilibrated FTC with physiological solution and sufficient flow rate (min. 15 mL/min) at 37 °C
**** recalibration may be required

Measurement rangepH 5.5 - 8.5
Resolutionat pH = 7: ± 0.02 pH
Accuracy**at pH = 7: ± 0.05 pH
Driftat pH = 7: < 0.005 pH per day (sampling interval of 1 min.)
Measurement temperature rangefrom + 5 to + 50 °C
Response time (t90)***< 120 sec.
Properties*
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Cross sensitivityReduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
Cleaning procedure****Irradiation, Ethylene oxide (EtO)
CalibrationSSTs (sensor sticks) are pre-calibrated; single-point calibratin is recommended
Storage stability18 months provided the sensor is stored in the dark
T-cell formats Luer T-cell (delivered), inner diameter 5 mm, cell volume 0.3 mL;
1/4" x 1/4" (Qosina), cell volume 2.1 mL;
3/8" x 3/8" (Qosina), cell volume 4.6 mL;
1/2" x 1/2" (Qosina), cell volume 8.3 mL

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