Process Monitoring in Suspension-Adapted CHO Cell Cultures

Harry Abts and Sarina Arain;

BioProcess International Vol. 6 No. 1

Noninvasive Online Detection of pH and Oxygen

Suspension-adapted Chinese hamster ovary cell (CHO-S) cultures are widely used in biotechnological production of recombinant proteins. In fact, such special cell lines have become the standard for this type of biopharmaceutical production. The reasons for that include their fast reproduction, high protein expression rate compared with other eukaryotic cells and, above all, the glycosylation patterns generated by the cells. Efficient production of correctly processed proteins requires optimized cultivation parameters.