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How long do I need to equilibrate before starting a SDR measurement?
Absolute equilibration times depend on many factors in your measurement set-up (volume, temperature, incubator atmosphere, shaking, etc.). Therefore, we cannot give a standard time frame for equilibration.
We recommend using the SDR to watch the kinetics and find the time point of equilibration. When the control wells' signal is stable, you can either start a new measurement, or adjust your measurement temperature in case it is not exactly at the set value. For oxygen and pH, you can also perform a one-point adjustment to set the start values to the exact oxygen or pH values (please start a new measurement for one-point adjustment).