Real-Life CHO Cultivation in Single Use Bags

Stephan C Kaiser, Franziska Fietz, Nina Steiger and Dieter Eibl

IPT Issue 45 June 2013

A novel fibre optic conversion model which connects single-use sensors to conventional controllers could provide new readings using electrochemical signals. How will the device fare after the application of an endurance test? In a recent study, a prototype fibre optic device has been applied for monitoring cultivation parameters in suspension-adapted Chinese hamster ovary (CHO) cell culture. This device converts the reading of chemical optical sensors integrated in the cultivation bag into electrochemical signals, which are transferred to a conventional controller. CHO cells were successfully cultivated for five days in this set-up. Although the culture showed untypical growth, correct functioning of the prototype device throughout the whole cultivation period was verified even though operational errors occurred.


Select by industry

Presens TV

Watch tutorials, webinars and informative videos about PreSens optical sensor systems.

All videos