Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
Online Carbon Dioxide Monitoring in Perfusion Systems
CO2 Flow-Through Cell FTC-CD1
The FTC-CD1 consists of a miniaturized optical carbon dioxide sensor integrated in a glass tube with 7 mm outer diameter. A customized holder fixes the polymer optical fiber for sensor read out. The cell is autoclavable.
- Online monitoring in perfusion systems
- Contactless measurements through the cell wall
- Measurement range from 10 - 250 hPa pCO2 (8 ... 180 mmHg)
- No consumption of carbon dioxide
Applications
PreSens CO2 Sensors as an Alternative to Off-line Sampling
PreSens CO2 sensors were evaluated for monitoring the cultivation cells in a 3-L bioreactor. The sensors show excellent long-term stability over a period of 10 days during cell cultivation of CHO cells with an initial seed density of 400,000 cells/mL. The readings of the PreSens CO2 sensor offer the possibility to continuously monitor the CO2 concentration between two off-line measurements.
Rick Baggio, Millipore Corporation, Bedford, MA, USA, Poster Presentation at ESACT2009, Dublin
Technical
| Specifications* | |
|---|---|
| *provided pCO2 spots are used without further handling in physiological solutions **after multipoint calibration ***in a carbon dioxide incubator with 100 % rel. hum. at 5 % CO2; measurement interval of 1 min. | |
| Measurement range | 1 - 25 % CO2 at atmospheric pressure (1013.15 hPa) 10 - 250 hPa pCO2 8 - 180 mmHg pCO2 |
Resolution at 20 °C | ± 0.06 % at 2 % CO2 ± 0.15 % at 6 % CO2 ± 0.5 mmHg at 15 mmHg pCO2 ± 1.2 mmHg at 45 mmHg pCO2 |
| Accuracy** | ± 5 % of reading or 0.2 % (1.5 mmHg); whichever is higher |
| Drift at 37 °C*** | Typically < 5 % of reading per week |
| Measurement temperature range | from + 15 to + 45 °C |
| Response time (t90) at 20 °C | < 3 min. for change from 2 % to 5 % (15 mmHg - 38 mmHg) pCO2 |
| Properties* | |
| Compatibility | Aqueous Solutions, pH 4 - 9 |
| Cross-sensitivity | Optical pCO2 sensors Display reduced cross-sensitivity to ionic strength (salinity); acetic acid, SO2, HCl vapours |
| Stability | pCO2 sensors do not stand: organic solvents, pH above 10 or below 4 |
| Cleaning procedure | Depends on the sensor type used - please ask our experts |
| Calibration | pCO2 spots are pre-calibrated, re-calibration is possible Beta-irradiated or autoclavable pCO2 sensors available |
| Storage stability | 12 months provided the pCO2 sensor is stored in its original package |
Related products
Resources
FAQs
Can I measure CO2 also in a gas phase or only in liquid phase?
Do I measure CO2 or HCO3-?
Does the pH of the sample affect my CO2 sensor reading?
How do I import data into excel from a text file containing measurement data?
What happens if the temperature changes while measuring CO2?
What is the operating principle of the CO2 sensor?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?