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Measuring pH with High Spatial Resolution
Needle-type pH Microsensor NTH-HP5
The NTH-HP5 is designed for measuring in small volumes. The sensor is based on a 140 μm silica fiber which enables integration into manifold small scale environments. With its protective syringe needle housing the NTH-HP5 can easily penetrate tissue, septum rubber or packaging materials. After penetration the sensor tip is extended for measurements.
- High spatial resolution
- Measurement in smallest volumes
- Profiling of pH gradients
- Integration into plant and animal tissue
- Optimized for culture media and physiological solutions
Applications
pH Measurement in Plants & Animals
pH Microsensors can be implemented in plant tissue or even in small animals. New insights in physiological aspects can be obtained. Due to the small size of the probes only a minimal disturbance will occur.
pH Measurement in Small Volumes
Due to the small dimension of the probe, pH measurements can be done in very small volumes - even in microtiter plates of a higher format like 384 or 1536. No need for reference electrodes - a real step forward, especially in small volumes. Of course, the measurement is independent of electromagnetical fields - this even allows measuring in NMR spectrometers.
Technical
| Specifications* | |
|---|---|
| *provided pH sensors are used without further handling in physiological solutions **calibration and following measurements in the same conditions / system; equilibrated sensor kept in well stirred solution at + 37 °C | |
| Measurement range | 5.5 - 8.5 pH |
| Resolution | at pH = 7: ± 0.02 pH |
| Accuracy** | at pH = 7: ± 0.1 pH with sensor calibration |
| Drift | at pH = 7: < 0.05 pH per day (sampling interval of 1 min.) |
| Measurement temperature range | from + 5 to + 50 °C |
| Response time (t90)** | at 25 °C: < 30 sec. |
| Properties* | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2- 10 |
| No cross-sensitivity | Electrical fields, proteins |
| Cross-sensitivity | Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere |
| Sterilization procedure | Ethylene oxide (Et0), recalibration recommended |
| Cleaning procedure | Water, Acrylan, pepsin solution |
| Calibration | pH sensors are pre-calibrated; recalibration is possible |
Related products
Resources
Publications
pH Monitoring of Urine and Tumor Microenvironments in Rats
Simultaneous Monitoring of pH and Oxygenation in Microvolumes of Blood
The protective role of proton‑sensing TDAG8 in the brain injury in a mouse ischemia reperfusion model
Luciferin-Bioinspired Click Ligation Enables Hydrogel Platforms with Fine-Tunable Properties for 3D Cell Culture
Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model
Early pH Changes in Musculoskeletal Tissues upon Injury - Aerobic Catabolic Pathway Acitvity Linked to Inter-Individual Differences in Local pH
Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy
The choroid plexus sodium-biocarbonate cotransporter NBCe2 regulates mouse cerebrospinal fluid pH
Migration capacity and viability of human primary osteoblasts in synthetic three-dimensional bone scaffolds made of tricalciumphosphate
Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in blood microvolumes
A hierarchy of factors influence discontinuous gas exchange in the grasshopper Paracinema tricolor (Orthoptera: Acrididae)
FAQs
Can I measure inside a tissue with fiber optic microsensors?
How can I prevent oxygen or pH microsensors from breaking when measuring profiles across tissue?
How do I import data into excel from a text file containing measurement data?
How does temperature affect my pH measurement?
How often will I have to exchange the microsensor?
How to prepare buffers of constant ionic strength and calibrate optical pH sensors?
Is there a recoating service for the needle-type microsensors?
The needle tip of the microsensor is vibrating or not moving smoothly when I try to insert it in the sample with the Manual Micromanipulator. What can I do?
What is the correct way for handling an oxygen or pH microsensor?
What is the difference between tapered and flat-broken microsensor tips?
What is the time of delivery?
Which buffers can be used for calibration of chemical optical pH sensors?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Why does my pH sensor not work in tap water?