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Luer Lock Adapter with Sensor for Customized Integration

pH Sensor Stick SST-HP5-US

This Luer Lock adapter is equipped with a miniaturized pH Sensor Spot type HP5 (measurement range pH 5.5 - 8.5). A polymer optical fiber is attached to the Sensor Stick and connects it to a pH meter. This way continuous, non-invasive pH measurements can be performed. The pH Sensor Stick can easily be integrated in customer specific flow-through cells or experimental set-ups via Luer connectors. The SST-HP5-US is independent of flow velocity or electromagnetic fields and due to its small size it can be implemented in many set-ups where other pH measurement equipment simply does not fit.

  • Non-invasive pH measurements in a range of pH 5.5 - 8.5
  • Small size
  • Easy connection via Luer Lock
  • For customized integration & pefusion cell culture monitoring
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Applications

pH Monitoring in Liver Cell Bioreactor

An important aspect of efficient liver cell bioreactors is the automated regulation of physio-chemical culture parameters. A non-invasive pH regulation device for a perfusion bioreactor has been developed. The high performance of the system is based on one of our chemical optical flow-through cells for pH detection and its combination with precision mass-flow controllers for gas. The new controller allows long time stable and contamination free online pH regulation in complex bioreactor systems - an important technical contribution for future clinical applications.

Application Note by Marco Decker: pH Regulation for a Liver Cell Bioreactor


Online Measurement in Perfusion Systems

Beta-irradiated and pre-calibrated pH and DO flow-through sensors can be integrated into perfusion systems. This allows easy control of process parameters in perfusion reactors. Typically Luer connectors are used, though different sizes for larger or smaller flow rates are available.


Technical

Specifications*
*provided the pH Sensor Stick is used without further handling in physiological solution
**after single-point calibration at pH = 7
***equilibrated Sensor Stick with physiological solution and sufficient flow rate (min. 15 mL/min) at 37 °C)
****recalibration may be required
Measurement rangepH 5.5 - 8.5
Resolutionat pH = 7: ± 0.02 pH
Accuracy**at pH = 7: ± 0.05 pH
Driftat pH = 7: < 0.005 pH per day (sampling interval of 1 min.)
Measurement temperature rangeFrom + 5 °C to + 50 °C
Response time (t90)***< 120 sec.
Properties
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Cross sensitivityReduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
Sterilization procedures****Irradiation, Ethylene oxide (EtO)
CalibrationSSTs are pre-calibrated; single-point adjustment is recommended
Storage stability18 months provided the sensor is stored in the dark

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