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Measure pH in Real Time in 96 Well Plates
PhoxyPlate 96 P
The PhoxyCube 96 P are 96-well pates with optical pH sensors integrated at the bottom of each well. Placed on the PhoxyCube readout unit the pH in the wells can be monitored in real time. The non-invasive measurement principle is based on PreSens imaging technology. The whole PhoxyCube system with PhoxyPlates can be placed in an incubator and is therefore ideally suited for high-throughput screenings, cell culture and toxicity screenings. See critical effects immediately, optimize your culture conditions, never miss the slightest change with PhoxyCube!
- Non-invasive pH monitoring in each well
- Ready-to-use disposable
- Visualization of kinetics in real time
- Use in incubator
- Ideal for screenings, cell cultivation, toxicity tests & more
Applications
Simultaneous O2 & pH Monitoring in Cell Culture
Monitoring oxygen and pH levels is critical in cell culture. Changes in O2 concentration or pH can lead to metabolic stress and alter cell functions. With the PhoxyCube system these two parameters can be measured in real time; optimal culture conditions can be ensured and inaccurate experiment results avoided.
Dose-Response Study in Pharmacological Research
The measurement of dose-response relationships is essential for understanding the effects of pharmacological agents on cellular functions. Changes in cell metabolism also show in changes in the cells’ oxygen uptake rate. With the PhoxyCube system and the PhoxyPlate 96 O highly parallelized oxygen measurements and dose-response studies can easily be performed. Adverse effects will show immediately in the online oxygen measurement data.
Respiration Measurements with PhoxyCube
PhoxyCube is the ideal tool for respiration measurements and research. Oxygen can be monitored in a multitude of samples simultaneously over time. Whether it is germination in plant seeds or respiration of small aquatic organisms, whatever your research subject is, with PhoxyCube any changes in oxygen consumption will be visible immediately.
Technical
| Specifications | |
|---|---|
| Measurement range* | 5.5 - 8.0 pH |
| Precision*,** | ± 0.01 pH at pH = 7 |
| Resolution* | ± 0.01 pH |
| Drift* | < 0.1 pH units within one week (sampling interval 10 min) |
| Response time (t90) at 37 °C | < 60 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 9 |
| Cross-sensitivity | Reduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere |
| Cleaning procedure | No cleaning; single-use product |
| General sensor operating temperature range*** | From + 5 °C to + 45 °C |
| Calibration | Batch-calibration values for 37 °C and buffer with physiological osmolality provided; re-calibration is possible, one-point adjustment is advised for accurate measurements |
| Plate type and format | 96-well Corning (flat bottom) |
| Irradiation | Beta-irradiated |
| Storage stability**** | 24 months provided the sensor is stored in the dark in its original package |
| *in physiological solutions at 37 °C **typical precision (average of all measurement positions), precision according to ISO 5725-1 ***Calibration needs to be performed for the respective temperature ****storage stability tested at + 20 °C ± 5 °C | |
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Resources
Manuals
Software
PreSens PhoxyCube Reader Software v1.0.1 - Windows 10, 11