Robust Microsensor for pH Profiling in Semi-solids

Profiling pH Microsensor PM-HP5

The pH Profiling Microsensor can be used for all profiling applications in semi-solid substrates, such as sediments, microbial mats or biofilms. The PM-HP5 is the most robust version of PreSens pH Microsensors and has a close-fitting fiber guidance and mechanical interlock for precise vertical localization of the measurement tip. The sensor tip is extendable with a turning mechanism.

  • Close-fitting fiber guidance
  • Mechanical interlock
  • High spatial resolution
  • Measurement in smallest volumes
  • Profiling of pH gradients
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Microprofiling in Biological & Environmental Research

The different types of oxygen and pH microsensors allow e. g. measurements in smallest sample volumes or inside tissue. The micromanipulators should be applied whenever it is necessary to insert the microsensor safely into semi-solid samples and when exact localization and stabilization of the microsensor tip within the sample is required. Using the safe-insert function the microsensor tip can be securely inserted and localized at the exact position where you want to conduct your measurements.

Microsensor Measurements in Medical & Life Science Research

PreSens microsensors are ideal tools for medical and life science research, as they allow for precise on the spot measurement and microprofiling inside tissue constructs. The Manual Micromanipulator is the indispensable equipment in these applications for exact localization of the microsensor inside the sample and microprofiling in step sizes down to 10 μm. PreSens needle-type microsensors are used in many tissue engineering applications.

Microprofiling of Sediments & Biofilms

Together with the specially designed PreSens Profiling Microsensors (PM) the Automated Micromanipulator is the ideal tool for oxygen measurements in sediment and biofilm applications. With a free choice of step zones, travel velocities and wait times different layers inside the sample can be monitored and assessed in step sizes down to 10 μm. The software visualizes the online measurements, so you can follow gradients and identify boundaries immediately while the sensor is automatically moved inside the sample.


*provided pH sensors are used without further handling in physiological solutions
**calibration and following measurements in the same conditions / system; equilibrated sensor kept in well stirred solution at + 37 °C
Measurement range 5.5 - 8.5 pH
Resolution at pH = 7: ± 0.02 pH
Accuracy** at pH = 7: ± 0.1 pH with sensor calibration
Drift at pH = 7: < 0.05 pH per day (sampling interval of 1 min.)
Measurement temperature range from + 5 to + 50 °C
Response time (t90)** at 25 °C: < 30 sec.
Compatibility Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
No cross-sensitivity Electrical fields, proteins
Cross-sensitivity Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
Sterilization procedure Ethylene oxide (Et0), recalibration recommended
Cleaning procedure Water, Acrylan, pepsin solution
Calibration pH sensors are pre-calibrated, recalibration is possible

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