Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
Contactless measurements & in-process monitoring
CO2 Sensor Spot SP-CD1
The CO2 sensor spots measure the partial pressure of dissolved carbon dioxide. The spots are fixed on the inner surface of glass or transparent plastic vessels. Measurements are then taken contactless from the outside through the transparent container material.
- Re-usable & disposable sensors
- Online monitoring
- Non-invasive & non-destructive measurement
- Measurement range from 10 - 250 hPa pCO2 (8 ... 180 mmHg)
- No consumption of carbon dioxide
- Measures carbon dioxide in liquids
- Beta-irradiated and autoclavable sensors available
Applications
Biotechnology: Carbon Dioxide Monitoring in Process Development
In bioprocess development & production, monitoring dissolved CO2 in addition to pH and O2 provides valuable information. It ensures stress-free cultivation conditions during mammalian cell culture (e.g. CHO cell-line). Constant pH and / or oxygen supply and sufficient nutrition levels are key parameters to optimize yield. Thereby, excess CO2 can act as a stressor or even a toxin to the culture and has to be controlled. The chemical optical CO2 sensors can enhance performance of process monitoring during cultivation in disposables, leading to process optimization.
PreSens CO2 Sensors as an Alternative to Off-line Sampling
PreSens CO2 sensors were evaluated for monitoring the cultivation cells in a 3-L bioreactor. The sensors show excellent long-term stability over a period of 10 days during cell cultivation of CHO cells with an initial seed density of 400,000 cells/mL. The readings of the PreSens CO2 sensor offer the possibility to continuously monitor the CO2 concentration between two off-line measurements.
Rick Baggio, Millipore Corporation, Bedford, MA, USA, Poster Presentation at ESACT2009, Dublin
Technical
| Specifications* | |
|---|---|
* provided pCO2 spots are used without further handling in physiological solutions | |
Measurement range | 1 - 25 % CO2 at atmospheric pressure (1013.15 hPa) |
Resolution at + 20 °C | ± 0.06 % at 2 % CO2 |
Accuracy** | ± 5 % of reading or 0.2 % (1.5 mmHg); whichever is higher |
Drift at + 37 °C*** | typically < 5 % of reading per week |
Measurement temperature range | from + 15 to + 45 °C |
Response time (t90) | < 3 min. for change from 2 % to 5 % (15 mmHg - 38 mmHg) pCO2 |
Properties |
|
Compatibility | Aqueous solutions, pH 4 - 9 |
Cross-sensitivity | Optical pCO2 sensors display reduced cross-sensitivity to ionic strength (salinity); acetic acid, SO2, HCl vapours |
Stability | pCO2 sensors do not stand: organic solvents, pH above 10 or below 4 |
Cleaning procedure | Depends on the sensor type used - please ask our experts |
Calibration | pCO2 spots are pre-calibrated , re-calibration is possible |
Storage stability | 12 months provided the pCO2 sensor is stored in its original package |
Related products
Resources
Publications
Simultaneous pH, CO2 and O2 Measurements in Algae Photobioreactor
Advanced Respirometry with Chemical Optical Sensor Spots
Online in Situ Measurements of Soil CO2 Concentrations in Dependence of Ash Roots (Fraxinus excelsior L.)
Non-invasive Online CO2 Measurement with Chemical Optical CO2 Sensors
Impact of dCO2 on Process Parameters
In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture
Portable Measurement System for in situ Estimation of Oxygen and Carbon Fluxes of Submerged Plants
FAQs
Can I measure CO2 also in a gas phase or only in liquid phase?
Do I have to mount the sensors in the dark because it says "Protect from light" on the packing?
Do I measure CO2 or HCO3-?
Do the sensors work in turbid solutions?
Does the pH of the sample affect my CO2 sensor reading?
How do I import data into excel from a text file containing measurement data?
How many samples should I use to perform a batch calibration?
In which vessel can I integrate a sensor spot?
Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables?
Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot?
What happens if the temperature changes while measuring CO2?
What is the operating principle of the CO2 sensor?
What is the time of delivery?
Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)?
Which glue can I use to integrate optical sensor spots?
Which substances can interfere with the optical O2, pH and CO2 measurements?