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Online pH Monitoring in Microfluidic & Millifluidic Chips
pH SensorPlug
The pH SensorPlugs by PreSens enable online pH monitoring in millifluidic and microfluidic applications. With the appropriate chip and port design, the SensorPlugs can be integrated at one or several positions on your microfluidic device. An optical sensor is attached to an e.g. Mini Luer based plug, which can easily be integrated in your chip via the corresponding port. The plug is connected to a pH meter via a polymer optical fiber (1 mm diameter) and the sensor is read out non-invasively, so there is no risk of contamination. SensorPlugs are delivered beta irradiated and pre-calibrated so you can start your measurements right away. We offer customized SensorPlug formats as well as support for chip and port designs including the VisiSens 2D imaging system for preliminary evaluation of your chip. In order to obtain even more information about your process you can combine the pH SensorPlug with plugs for O2 and CO2 measurement. The monitoring with all SensorPlug types can be controlled from one PC with the PreSens Measurement Studio 2 software.
- Non-invasive pH monitoring in millifluidic & microfluidic chips
- Pre-calibrated sensors, plug & play
- Beta irradiated
- Compatible with PreSens Measurement Studio2, standard PreSens pH meters and EOMs
Applications
Cell Culture Microenvironment Monitoring
SensorPlugs offer the unique possibility to measure contactless directly inside the channel. This way the cells' undisturbed microenvironment can be monitored and investigated. Tumor cell research, the assessment of cell metabolism or cell signaling in organ-on-a-chip applications will profit from this information.
Reaction Monitoring in Microfluidics
Oxygen, pH and CO2 are important parameters in many chemical reactions. Direct monitoring in the microchannels can enable precise control of the reactions and deliver decisive information about reaction speed. Oxygen monitoring with SensorPlugs is a further step towards real Lab-on-a-Chip applications.
Technical
| Specifications* | |
|---|---|
| *provided the pH SensorPlugs are used without further handling in physiological solutions **calibration and following measurements in the same conditions / system; equilibrated sensor kept in well stirred solution at + 37 °C | |
| Measurement range | pH 5.5 - 8.5 |
| Resolution | at pH = 7: ± 0.01 pH |
| Accuracy** | at pH = 7: ± 0.05 pH (sensor spot calibration) at pH = 7: ± 0.10 pH (sensor batch calibration) |
| Measurement temperature range | From 0 to + 50 °C |
| Response time (t90)** | < 120 sec. |
| Compatible pH meters | pH-1 SMA HP5 with SMA-ST-Adapter |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Cross-sensitivity | Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere |
| Cleaning procedure | pH SensorPlugs are delivered beta-irradiated; a second irradiation or autoclaving is not recommended |
| Calibration | pH SensorPlugs are pre-calibrated |
| Plug type | Male Mini Luer fluid connector; customized plug formats available on request |
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Resources
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A Microfluidic Platform for Real-Time Non-Invasive Monitoring of Microglia Polarization
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Microfludic Platform for Examination of Effect of Chewing Xylitol Gum on Salivary pH, O2, and CO2
Microfluidic Platform for Examination of Effect of Chewing Xylitol Gum on Salivary pH, O2, and CO2