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Online O2 & pH Monitoring in Cell Culture

Cell Culture Flask with Integrated Oxygen and pH Sensor CFS-HP5-PSt3

This plastic cell culture flask with integrated sensors is available for different growth areas. It is delivered irradiated and pre-calibrated. The CFS-HP5-PSt3 can be read out with the SFR Shake Flask Reader or SFR vario in combination with the T-Flask Adapter.

  • Ready-to-use
  • Pre-calibrated sensors
  • Contactless measurement
  • For cell culture
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Technical

SpecificationsOxygenpH*
* provided Cell Culture Flasks are used without further handling in physiological solution
** at 100 rpm & in cell culture media
Measurement range0 - 100 % O25.5 - 8.0 pH
Resolution± 0.01 % O2 at 0.21 % O2± 0.01 pH at pH = 7**
Accuracy

± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2

± 0.1 pH at pH = 7 with one-point adjustment
± 0.2 pH at pH = 7 with pre-calibration
Drift< 0.01 % O2 per day
(sampling interval of 1 min.)
< 0.01 pH per day
(sampling interval of 1 min.)
Measurement temperature rangefrom + 5 to + 50 °C
Response time (t90)**at 25 °C: < 60 sec.
Properties
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Cross-sensitivityTypically no cross-sensitivity in culture mediaReduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
CalibrationSensor flasks are delivered irradiated.
Pre-calibrated

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