Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
Contactless measurements & in-process monitoring
CO2 Sensor Spot SP-CD1
The CO2 sensor spots measure the partial pressure of dissolved carbon dioxide. The spots are fixed on the inner surface of glass or transparent plastic vessels. Measurements are then taken contactless from the outside through the transparent container material.
- Re-usable & disposable sensors
- Online monitoring
- Non-invasive & non-destructive measurement
- Measurement range from 10 - 250 hPa pCO2 (8 ... 180 mmHg)
- No consumption of carbon dioxide
- Measures carbon dioxide in liquids
- Beta-irradiated and autoclavable sensors available
Biotechnology: Carbon Dioxide Monitoring in Process Development
In bioprocess development & production, monitoring dissolved CO2 in addition to pH and O2 provides valuable information. It ensures stress-free cultivation conditions during mammalian cell culture (e.g. CHO cell-line). Constant pH and / or oxygen supply and sufficient nutrition levels are key parameters to optimize yield. Thereby, excess CO2 can act as a stressor or even a toxin to the culture and has to be controlled. The chemical optical CO2 sensors can enhance performance of process monitoring during cultivation in disposables, leading to process optimization.
PreSens CO2 Sensors as an Alternative to Off-line Sampling
PreSens CO2 sensors were evaluated for monitoring the cultivation cells in a 3-L bioreactor. The sensors show excellent long-term stability over a period of 10 days during cell cultivation of CHO cells with an initial seed density of 400,000 cells/mL. The readings of the PreSens CO2 sensor offer the possibility to continuously monitor the CO2 concentration between two off-line measurements.
Rick Baggio, Millipore Corporation, Bedford, MA, USA, Poster Presentation at ESACT2009, Dublin
* provided pCO2 spots are used without further handling in physiological solutions
1 - 25 % CO2 at atmospheric pressure (1013.15 hPa)
Resolution at + 20 °C
± 0.06 % at 2 % CO2
± 5 % of reading or 0.2 % (1.5 mmHg); whichever is higher
Drift at + 37 °C***
typically < 5 % of reading per week
Measurement temperature range
from + 15 to + 45 °C
Response time (t90)
< 3 min. for change from 2 % to 5 % (15 mmHg - 38 mmHg) pCO2
Aqueous solutions, pH 4 - 9
Optical pCO2 sensors display reduced cross-sensitivity to ionic strength (salinity); acetic acid, SO2, HCl vapours
pCO2 sensors do not stand: organic solvents, pH above 10 or below 4
Depends on the sensor type used - please ask our experts
pCO2 spots are pre-calibrated , re-calibration is possible
12 months provided the pCO2 sensor is stored in its original package
The pCO2 mini is a precise fiber optic CO2 meter with...
The electro-optical-module EOM-CO2-mini is a precise OEM...
Polymer Optical Fiber POF
A polymer optical fiber (POF) is needed to transfer...
Stick-On Adapter SOA
The stick-on adapter is used to attach the polymer optical...
Adapter for Round Containers ARC
The Adapter for Round Containers is an adjustable...