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Measuring Low pH with High Spatial Resolution
Needle-type pH Microsensor NTH-HP5-ST-low
The NTH-HP5-ST-low is designed for measuring in small volumes at low pH (4 – 7 pH). The sensor is based on a 250 μm plastic fiber, which enables integration into manifold small-scale environments and is also very resistant against damage or breaking. With its protective syringe needle housing the NTH-HP5-ST-low can easily penetrate tissue, septum rubber or packaging materials. After penetration the sensor tip is extended for measurements.
- Robust plastic fiber
- For measurements in low pH range (4 – 7 pH)
- High spatial resolution
- Measurement in smallest volumes
- Integration into plant and animal tissue
- Optimized for culture media and physiological solutions
Applications
pH Measurement in Plants & Animals
pH Microsensors can be implemented in plant tissue or even in small animals. New insights in physiological aspects can be obtained. Due to the small size of the probes only a minimal disturbance will occur.
pH Measurement in Small Volumes
Due to the small dimension of the probe, pH measurements can be done in very small volumes - even in microtiter plates of a higher format like 384 or 1536. No need for reference electrodes - a real step forward, especially in small volumes. Of course, the measurement is independent of electromagnetical fields - this even allows measuring in NMR spectrometers.
Technical
| Specifications* | |
|---|---|
| *provided pH sensors are used without further handling in physiological solutions **equilibrated sensor kept in well stirred solution at + 37 °C | |
| Measurement range | 4 - 7 pH |
| Resolution | at pH = 6: ± 0.02 pH |
| Drift | at pH = 6: < 0.1 pH per day (sampling interval of 1 min.) |
| Measurement temperature range | from + 5 to + 50 °C |
| Response time (t90)** | at + 37 °C: < 30 sec. |
| Properties* | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), pH 2 - 10 |
| No cross-sensitivity | Electrical fields, proteins |
| Cross-sensitivity | Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere |
| Disinfection procedure | One-time disinfection with EtOH (70 % v/v) or isopropanol (100 % v/v) |
| Cleaning procedure | Buffer, water, pepsin solution |
| Calibration | One-point or multi-point calibration procedure supported by PreSens Measurement Studio 2 software |
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Resources
FAQs
Can I measure inside a tissue with fiber optic microsensors?
How often will I have to exchange the microsensor?
How to prepare buffers of constant ionic strength and calibrate optical pH sensors?
Is there a recoating service for the needle-type microsensors?
The needle tip of the microsensor is vibrating or not moving smoothly when I try to insert it in the sample with the Manual Micromanipulator. What can I do?
What is the correct way for handling an oxygen or pH microsensor?
Which buffers can be used for calibration of chemical optical pH sensors?
Why does my pH sensor not work in tap water?
Manuals
Brochures
Software
PreSens Measurement Studio 2 version 4.0.0 - Windows 10/11
PreSens Profiling Studio version 2.0.0 - Windows 10/11