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Measurements Under Real Conditions with Pre-Calibrated Sensors
pH Sensor Spots SP-HP5
The SP-HP5 allow online monitoring and are optimized for physiological solutions and culture media. The spots are mounted in transparent vessels made of glass or plastic and then read out contactless from the outside - through the container wall. The sensors are pre-calibrated and ready-to-use.
- Online monitoring without sampling
- Contactless measurements through the vessel wall
- Pre-calibrated
- Applicable from microliter to production scale
- Optimized for culture media and physiological solutions
Applications
Bioprocess Development: pH Monitoring in Shake Flasks
The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used. The combination with non-invasive oxygen sensors in shake flasks provides new insights into metabolic activity and changes in metabolic pathways.
Technical
| Specifications* | |
|---|---|
* provided pH sensors are used without further handling in physiological solutions | |
| Measurement range | 5.5 - 8.5 pH |
| Resolution | at pH = 7: ± 0.01 pH |
| Accuracy** | at pH = 7: ± 0.05 sensor spot calibration at pH = 7: ± 0.10 sensor batch calibration |
| Drift | at pH = 7: < 0.005 pH per day (sampling interval of 1 min.) |
| Measurement temperature range | from + 5 to + 50 °C |
| Response time (t90)** | < 120 sec. |
| Properties* | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Cross-sensitivty | Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere |
| Cleaning procedure | pH spots are delivered either beta-irradiated or untreated, a second irradiation or ethylene oxide treatment is not recommended |
| Sterilization procedure*** | Irradiation Ethylene oxide (EtO) Autoclaving (one time) |
| Calibration | pH spots are pre-calibrated; re-calibration is possible |
Related products
Resources
Publications
Fungal Metabolite Screening Inside a Microfluidic Bioreactor
Advanced Respirometry with Chemical Optical Sensor Spots
Online pH and DO Measurements in Microcarrier-Based hMSC Cultivations
Recommendations for process engineering characterisation of single-use bioreactors and mixing systems by using experimental methods (2nd Edition)
SCREENED: A Multistage Model of Thyroid Gland Function for Screening Endocrine-Disrupting Chemicals in a Biologically Sex-Specific Manner
Microbioreactor for lower cost and faster optimisation of protein production
Bioreactor-manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies
Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)
Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes
Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations
New Milliliter-Scale Stirred Tank Bioreactors for the Cultivation of Mycelium Forming Microorganisms
Miniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coli
Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16
Milliliter-Scale Stirred Tank Reactors for the Cultivation of Microorganisms
Growth and recombinant protein expression with Escherichia coli in different batch cultivation media
Process performance of parallel bioreactors for batch cultivation of Streptomyces tendae
A New Microfluidic Concept for Parallel Operated Milliliter-Scale Stirred Tank Bioreactors
Reaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicum
FAQs
Can I use ethanol for disinfecting non-invasive pH sensors?
Do I have to mount the sensors in the dark because it says "Protect from light" on the packing?
Do the sensors work in turbid solutions?
How can I clean non-invasive pH sensors?
How do I import data into excel from a text file containing measurement data?
How does temperature affect my pH measurement?
How many samples should I use to perform a batch calibration?
How to prepare buffers of constant ionic strength and calibrate optical pH sensors?
In which vessel can I integrate a sensor spot?
Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables?
Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot?
What do I have to consider when autoclaving pH Sensor Spots?
What is the time of delivery?
Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)?
Which buffers can be used for calibration of chemical optical pH sensors?
Which glue can I use to integrate optical sensor spots?
Which side of the pH sensor spot should face the medium?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Why does my pH sensor not work in tap water?