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Online Monitoring in Perfusion Systems with Single-use Sensor
Single-Use pH Flow-Through Cell FTC-SU-HP5-S
A miniaturized optical pH sensor is attached to a color coded stick, which can be attached to flow-through cells of different size and shape, according to requirements. A polymer optical fiber connects the sensor inside the flow-through cell to the pH meter. The single-use T-cell of the FTC-SU-HP5-S is made of polycarbonate and all parts come beta-irradiated.
- Single-use flow-through cell
- Precise online monitoring of pH in perfusion systems
- Different sizes and shapes for various flow rates
- Easy connection to external tubing
- Customized connectors are available, e. g. CPC
Applications
pH Monitoring in Liver Cell Bioreactor
An important aspect of efficient liver cell bioreactors is the automated regulation of physio-chemical culture parameters. A non-invasive pH regulation device for a perfusion bioreactor has been developed. The high performance of the system is based on one of our chemical optical flow-through cells for pH detection and its combination with precision mass-flow controllers for gas. The new controller allows long time stable and contamination free online pH regulation in complex bioreactor systems - an important technical contribution for future clinical applications.
Application Note by Marco Decker: pH Regulation for a Liver Cell Bioreactor
Online Measurement in Perfusion Systems
Beta-irradiated and pre-calibrated pH and DO flow-through sensors can be integrated into perfusion systems. This allows easy control of process parameters in perfusion reactors. Typically Luer connectors are used, though different sizes for larger or smaller flow rates are available.
Technical
| Specifications* | |
|---|---|
| Measurement range | 5.5 - 8.5 pH |
| Resolution | at pH = 7: ± 0.01 pH |
| Accuracy** | at pH = 7: ± 0.05 pH sensor spot calibration at pH = 7: ± 0.10 pH sensor batch calibration |
| Drift | at pH = 7: < 0.005 pH per day (sampling interval of 1 min.) |
| Measurement temperature range | from + 5 to + 50 °C |
| Response time (t90)** | < 120 sec. |
| Properties* | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2- 10 |
| Cross-sensitivity | Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere |
| Cleaning procedure*** | Irradiation, Ethylene oxide (EtO) |
| Calibration | FTCs are pre-calibrated; single-point calibration is recommended |
| T-cell formats | Luer T-cell (delivered), inner diameter 5 mm, cell volume 0.3 mL; 1/4" x 1/4" (Qosina), cell volume 2.1 mL; 3/8" x 3/8" (Qosina), cell volume 4.6 mL; 1/2" x 1/2" (Qosina), cell volume 8.3 mL |
* provided pH sensors are used without further handling in physiological solutions | |
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FAQs
How do I import data into excel from a text file containing measurement data?
How does temperature affect my pH measurement?
How to prepare buffers of constant ionic strength and calibrate optical pH sensors?
What is the time of delivery?
Which buffers can be used for calibration of chemical optical pH sensors?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Why does my pH sensor not work in tap water?