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Miniaturized Oxygen Sensor Integrated in a Flow-Through Cell
Autoclavable O2 Flow-Through Cell FTC-PSt3-YAU
The FTC-PSt3-YAU is a miniaturized chemical optical oxygen sensor integrated in a flow-through cell. A glass tube with an inner diameter of 2 mm is coated with the sensor at its inner wall. The FTC is connected to the oxygen meter with an optical fiber. The volume for liquid inside the FTC is about 100 microliter. The standard flow-through cell can be easily connected via Luer-Lock adapters to external tubings.
- Online oxygen measurements in perfusion systems
- No consumption of oxygen
- Signal independent of flow velocity
- Autoclavable
Applications
In-line Oxygen Monitoring in 3D Cell Culture
Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. PreSens flow-through cells with integrated chemical optical sensors can be incorporated in the construct inlet and outlet and allow in-line measurement of oxygen tension.
Application Note by R. Santoro, et al.: Perfusion Culture of Cell Seeded 3D Scaffolds for Tissue Engineering
Technical
| Specifications | Gaseous & Dissolved O2 | Dissolved O2 |
|---|---|---|
| *after two-point calibration as described in the manual | ||
| Measurement range | 0 – 100 % O2 0 – 1000 hPa | 0 – 45 mg/L 0 – 1400 µmol/L |
| Limit of detection | 0.03 % oxygen | 15 ppb |
| Resolution | ± 0.01 % O2 at 0.21 % O2 | ± 0.004 mg/L at 0.091 mg/L |
| Accuracy* | ± 0.4 % O2 at 20.9 % O2 ± 0.05 % O2 at 0.2 % O2 | |
| Drift | < 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen) | |
| Measurement temperature range | from 0 to + 50 °C | |
| Response time (t90) | < 6 sec. | < 40 sec. |
| Properties | ||
| Compatibility | Aqueous solutions, ethanol, methanol | |
| No cross-sensitivity | pH 1 – 14 | |
| Cross-sensitivity | Organic solvents, such as acetone, toluene, chloroform or methylene chloride Chlorine gas | |
| Sterilization procedure | Steam sterilization Ethylene oxide (EtO) | |
| Cleaning procedure | 3 % H2O2 Acidic agents (HCl, H2SO4), max. 4 – 5 % | |
| Calibration | Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment | |
| Storage stability | 24 months provided the sensor material is stored in the dark | |
Related products
Resources
Publications
Perfusion Culture of Cell-seeded 3D Scaffolds for Tissue Engineering
Monitoring Oxygen in Perfused Muscular Tissue Using Flow-Through Cells FTC-PSt3
Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions
Computational evaluation of oxygen and shear stress distributions in 3D perfusion culture systems: Macro-scale and micro-structured models
Online oxygen measurements in ex vivo perfused muscle tissue in a porcine model using dynamic quenching methods
On-line monitoring of oxygen as a non-destructive method to quantify cells in engineered 3D tissue constructs
Assessing viability of extracorporeal preserved muscle transplants using external field stimulation: a novel tool to improve methods prolonging bridge-to-transplantation time
FAQs
How can I convert an oxygen value into a different oxygen unit?
How can I prepare the calibration solutions cal0 and cal100 for oxygen sensors?
How do I import data into excel from a text file containing measurement data?
How do I verify whether the oxygen sensor is giving correct readings (performance proof)?
How does an oxygen sensor work?
How does salinity affect the oxygen measurement?
How does temperature affect the oxygen measurement?
The Stern-Volmer-equation
What are the response times for the oxygen sensors based on the 2 mm fiber like non-invasive oxygen sensors and oxygen probes?
What is the maximum flow rate and pressure for your oxygen flow-through cells?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Manuals
Brochures
Software
PreSens Datamanager version 2.0.0.57 - Windows XP/Vista/7/8
PreSens Oxygen Calculator version 3.1.1 - Windows 7/8/10