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Trace Oxygen Measurements in Perfusion Systems

Autoclavable O2 Flow-Through Cell FTC-PSt6-YAU

The FTC-PSt6-YAU is a miniaturized trace oxygen sensor integrated in a flow-through cell. A glass tube with an inner diameter of 2 mm is coated with the sensor at its inner wall. The FTC is connected to the oxygen meter with an optical fiber. The volume for liquid inside the FTC is about 100 microliter. The standard flow-through cell can be easily connected via Luer-Lock adapters to external tubings.

  • Online oxygen measurements in perfusion systems
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Autoclavable

Applications

In-line Oxygen Monitoring in 3D Cell Culture

Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. PreSens flow-through cells with integrated chemical optical sensors can be incorporated in the construct inlet and outlet and allow in-line measurement of oxygen tension.

Application Note by R. Santoro, et al.: Perfusion Culture of Cell Seeded 3D Scaffolds for Tissue Engineering


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 5  % O2

0 – 41.4 hPa

0 – 2 mg/L

0 – 56.9 µmol/L
Limit of detection0.002 % oxygen

1 ppb

Resolution

± 0.0007 % O2 at 0.002 % O2
± 0.0015 % O2 at 0.2 % O2
± 0.007 hPa at 0.023 hPa

± 0.015 hPa at 2.0 hPa

± 0.0003 mg/L at 0.001 mg/L
± 0.0006 mg/L at 0.09 mg/L
± 0.010 µmol/L at  0.03µmol/L

± 0.020 µmol/L at 2.8 µmol/L
Accuracy*± 1 ppb or ± 3 % of the respective concentration; whichever is higher
Drift < 2 ppb within 30 days (sampling interval of 1 min./ at 0 % oxygen)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Steam sterilization
Ethylene oxide (EtO)

Cleaning procedure

3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 % – 5 %

CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 1 % and 2 % oxygen
Storage stability24 months provided the sensor material is stored in the dark

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