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Highly Parallelized Oxygen Monitoring in 96-well Microtiterplate

OxoPlate OP96C

The OxoPlate OP96C is a beta-irradiated 96-well flat bottom microplate made of polystyrene. An oxygen sensor is integrated on the bottom of each well. The sensors can be read out in parallel from the bottom side using a commercially available fluorescence reader.

  • Ready-to-use
  • User-defined batch calibration
  • For use with conventional microplate fluorescence readers
  • High throughput screening
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Technical

Specifications*
* in H2O dest. or oxygen-free water
Measurement range 0 - 30 % O2
Resolution ± 0.2 % O2at 0.21 % O2
±0.6 % O2 at 20.9 % O2
Precision ± 0.4 % O2 at 0.21 % O2
± 1.0 % O2 at 20.9 % O2
Drift < 0.04 % O2 per hour (sampling interval 1 min.)
Measurement temperature range from + 15 °C to + 45 °C
Response time (t90) at + 25 °C: < 30 sec.
Properties
Compatibility Aqueous solutions, ethanol (max . 10 % v/v), methanol (max. 10 % v/v), pH 2 – 10, 20 % (v/v) DSMO, DMF, acetone, THF, or dioxane in solution. (Calibration constants have to be determined using the respective solvent instead of water.)
Cross sensitivity Gaseous sulfur dioxide (SO2) and gaseous chlorine (Cl2); Changes in ionic strength
Calibration Needs to be calibrated once per batch
Disposables are delivered beta-irradiated
Plate type 96-well flat bottom plates (Greiner Microlon® 600)
Maximum filling volume 400 µL
Indicator filters 540 nm / 650 nm
Reference filters 540 nm / 590 nm
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