Highly Parallelized Oxygen Monitoring in 96-well Microtiterplate

OxoPlate OP96C

The OxoPlate OP96C is a beta-irradiated 96-well flat bottom microplate made of polystyrene. An oxygen sensor is integrated on the bottom of each well. The sensors can be read out in parallel from the bottom side using a commercially available fluorescence reader.

  • Ready-to-use
  • User-defined batch calibration
  • For use with conventional microplate fluorescence readers
  • High throughput screening
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* in H2O dest. or oxygen-free water
Measurement range0 - 30 % O2
Resolution± 0.2 % O2at 0.21 % O2
±0.6 % O2 at 20.9 % O2
Precision± 0.4 % O2 at 0.21 % O2
± 1.0 % O2 at 20.9 % O2
Drift< 0.04 % O2 per hour (sampling interval 1 min.)
Measurement temperature rangefrom + 15 °C to + 45 °C
Response time (t90) at + 25 °C: < 30 sec.
CompatibilityAqueous solutions, ethanol (max . 10 % v/v), methanol (max. 10 % v/v), pH 2 – 10, 20 % (v/v) DSMO, DMF, acetone, THF, or dioxane in solution. (Calibration constants have to be determined using the respective solvent instead of water.)
Cross sensitivityGaseous sulfur dioxide (SO2) and gaseous chlorine (Cl2); Changes in ionic strength
CalibrationNeeds to be calibrated once per batch
Disposables are delivered beta-irradiated
Plate type96-well flat bottom plates (Greiner Microlon® 600)
Maximum filling volume400 µL
Indicator filters540 nm / 650 nm
Reference filters540 nm / 590 nm

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