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Small Trace Oxygen Sensor for Contactless Measurement

Oxygen Sensor Spot SP-PSt8-NAU

These oxygen sensors measure the partial pressure of both dissolved and gasseous oxygen. They are fixed to the inner surface of glass or transparent plastic containers and can then be read out from the outside – through the vessel wall. The trace oxygen sensors type PSt8 can be used for measurements in trace oxygen range of 0 – 10 % O2 and have a detection limit of 0.007 % O2. The SP-PSt8-NAU is not suitable for autoclaving.

  • Non-invasive measurements through the vessel wall
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Measures oxygen in liquids as well as in gas phase
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Applications

Bioprocess Development: Oxygen Monitoring in Shake Flasks

O2 supply is one of the major issues in the cultivation of aerobic organisms. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of dissolved oxygen were missing, sufficient O2 supply is usually assumed. The non-invasive oxygen sensors in shake flasks now ensure oxygen supply and give new insights into metabolic activity.


Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range0 – 10 % O2
0 – 100 hPa

0 – 4.5 mg/L

0 – 140 µmol/L
Limit of detection0.007 % oxygen3 ppb
Resolution

± 0.002 % O2 at 0.008 % O2
± 0.06 % O2 at 2.5 % O2

± 0.7 ppb at 3 ppb
± 2.5 ppb at 1000 ppb

Accuracy at + 20 °C*± 3 ppb or ± 3 % rel.
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties  
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivity

Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas

Sterilization procedureEthylene oxide (EtO)
Cleaning procedure

3 % H2O2
Ethanol
Soap solution

CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and a second calibration value optimally between 2 % and 4 % oxygen
Storage stability60 months provided the sensor material is stored in the dark at room temperature

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