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pH Sensor Spots SP-HP5 Measurements Under Real Conditions with Pre-Calibrated Sensors

Measurements Under Real Conditions with Pre-Calibrated Sensors

pH Sensor Spots SP-HP5

The SP-HP5 allow online monitoring and are optimized for physiological solutions and culture media. The spots are mounted in transparent vessels made of glass or plastic and then read out contactless from the outside - through the container wall. The sensors are pre-calibrated and ready-to-use.

  • Online monitoring without sampling
  • Contactless measurements through the vessel wall
  • Pre-calibrated
  • Applicable from microliter to production scale
  • Optimized for culture media and physiological solutions
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Applications

Bioprocess Development: pH Monitoring in Shake Flasks

The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used. The combination with non-invasive oxygen sensors in shake flasks provides new insights into metabolic activity and changes in metabolic pathways.


Technical

Specifications*
* provided pH sensors are used without further handling in physiological solutions
** equilibrated sensor kept in well stirred solution at + 37 °C
Measurement range5.5 - 8.5 pH
Resolutionat pH = 7: ± 0.01 pH
Accuracyat pH = 7: ± 0.05 sensor spot calibration
at pH = 7: ± 0.10 sensor batch calibration
Driftat pH = 7: < 0.005 pH per day (sampling interval of 1 min.)
Measurement temperature rangefrom + 5 to + 50 °C
Response time (t90)**< 120 sec.
Properties*
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Cross-sensitivtyReduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
Cleaning procedurepH spots are delivered either beta-irradiated or untreated, a second irradiation or ethylene oxide treatment is not recommended
CalibrationpH spots are pre-calibrated; re-calibration is possible
Storage stability18 months provided the sensor is stored in the dark

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Resources

Publications

Advanced Respirometry with Chemical Optical Sensor Spots Online pH and DO Measurements in Microcarrier-Based hMSC Cultivations Recommendations for process engineering characterisation of single-use bioreactors and mixing systems by using experimental methods (2nd Edition) SCREENED: A Multistage Model of Thyroid Gland Function for Screening Endocrine-Disrupting Chemicals in a Biologically Sex-Specific Manner Microbioreactor for lower cost and faster optimisation of protein production Bioreactor-manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS) Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations New Milliliter-Scale Stirred Tank Bioreactors for the Cultivation of Mycelium Forming Microorganisms Miniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coli Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16 Milliliter-Scale Stirred Tank Reactors for the Cultivation of Microorganisms Growth and recombinant protein expression with Escherichia coli in different batch cultivation media Process performance of parallel bioreactors for batch cultivation of Streptomyces tendae A New Microfluidic Concept for Parallel Operated Milliliter-Scale Stirred Tank Bioreactors Reaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicum

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