Easy Integration into any Transparent Vessel

Self-adhesive pH Sensor Spot SP-HP5-SA

The new self-adhesive technology simplifies the integration of these sensor spots. They can be mounted in transparent glass or plastic vessels. The pH is measured non-invasively and non-destructively from the outside – through the vessel wall. Optimized for physiological solutions and culture media these sensors are especially suited for bioprocess development or pharmaceutical applications. They are available irradiated or untreated. These sensor spots come pre-calibrated and ready-to-use. One-time autoclaving is possible.

  • Simplified integration with new self-adhesive technology
  • Compatible with glass & nearly all plastic vessels
  • Pre-calibrated & ready-to-use
  • Online monitoring without sampling
  • Contactless measurements through the vessel wall
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Bioprocess Development: pH Monitoring in Shake Flasks

The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used. The combination with non-invasive oxygen sensors in shake flasks provides new insights into metabolic activity and changes in metabolic pathways.



* provided pH sensors are used without further handling in physiological solutions
** calibration and following measurements in the same conditions / system; equilibrated sensor kept in well stirred solution at 37 °C
*** recalibration is recommended

Measurement range 5.5 - 8.5 pH
Resolution at pH = 7: ± 0.01 pH
Accuracy** at pH = 7: ± 0.05 pH sensor spot calibration
at pH = 7: ± 0.10 pH sensor batch calibration
Drift at pH = 7: < 0.005 pH per day (sampling interval of 1 min.)
Measurement temperature range from + 5 to + 50 °C
Response time (t90)** < 120 sec.
Compatibility Aqueous solutions, methanol (max. 10 % v/v), pH 2 - 10
Cross-sensitivity Reduced to ionic strength (salinity); a high cnocentration of small fluorescent molecules in the visible range can interfere
Cleaning procedure*** pH spots are delivered either beta-irradiated or untreated; one-time autoclaving or Et0 treatment is possible;
a second irradiation or ethylene oxide treatment is not recommended
Calibration pH spots are pre-calibrated
Carrier materials Adhers to nearly all surfaces like glass, polystyrene, polyethylene, polymethacrylate (acrylic glass), polycarbonate. The sensor does NOT adhere to silicone surfaces!

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Are there any constraints when autoclaving glass or plastic vessels with integrated self-adhesive pH Sensor Spots SP-HP5-SA? Can I use ethanol for disinfecting non-invasive pH sensors? Can the customer integrate the optical sensor spots by himself / herself? Do I have to mount the sensors in the dark because it says "Protect from light" on the packing? Do the sensors work in turbid solutions? How can I clean non-invasive pH sensors? How do I import data into excel from a text file containing measurement data? How does temperature affect my pH measurement? How many samples should I use to perform a batch calibration? How to prepare buffers of constant ionic strength and calibrate optical pH sensors? In which vessel can I integrate a sensor spot? In which vessels can I integrate self-adhesive pH Sensor Spots SP-HP5-SA or SP-LG1-SA? Is the glue or the sensor spot biocompatible - which tests were done? Are there any studies on leachables and extractables? Is there a standard recommended distance to place the polymer optical fiber (POF) from the sensor spot? What do I have to consider when autoclaving pH Sensor Spots? What is the time of delivery? Where do I need sensor spot sizes different from the common diameter of 5 mm (approx. 0.2 inch)? Which buffers can be used for calibration of chemical optical pH sensors? Which flasks can be used with the SFR vario? Which glue can I use to integrate optical sensor spots? Which substances can interfere with the optical O2, pH and CO2 measurements? Which types and sizes of shake flasks with integrated sensors (SFS) are offered by PreSens? Why does my pH sensor not work in tap water?

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