Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
96-well Microplates with Integrated Chemical Optical pH Sensors
HydroPlate HP96U
The HydroPlate HP96U is a beta-irradiated polystyrene 96-well round bottom microplate. A pH sensor is immobilized on the bottom of each well. The sensors are read out from the bottom side using a commercially available fluorescence reader and the approx. wavelengths 485/540 nm and 485/620 nm.
- Ready-to-use
- For use with conventional fluorescence microplate readers
- High throughput screening
Applications
pH Monitoring in Dairy Starter Cultures
In order to get good and reproducible quality of dairy products suitable starter cultures have to be used. HydroPlates are an easy-to-use method to screen a large number of inoculum cultures as quality control for raw materials. Differences in inoculum type and size of cultures of Lactococcus lactis and Streptococcus thermophilus can be clearly detected, even though there are disturbances caused by particles and fluorophores contained in the medium. The recorded pH values are quite close to values measured offline with a pH electrode.
G. T. John, et al., 2003, Saarland University + Danisco, Saarbrücken, Germany
Technical
| Specifications | |
|---|---|
| * at 37 °C; performance dependent on the reader; provided constant ionic strength | |
| Measurement range | pH 5.0 - 8.0 |
| Resolution* | at pH = 7: ± 0.05 pH |
| Precision* | ± 0.2 pH at pH = 7 (sensor batch calibration) ± 0.1 pH at pH = 7 (sensor spot calibration) |
| Drift* | < 0.002 pH per hour (sampling interval 1 min.) at pH = 7 |
| Measurement temperature range | from + 15 to + 45 °C |
| Response time (t90)* | < 30 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Cross sensitivity | Changes in ionic strength Temperature |
| Calibration | Needs to be calibrated once per batch Beta-irradiated; not re-usable |
| Plate type | 96-well round bottom plates (Greiner Microlon® 600) |
| Working volume | 30 - 280 µL (max. filling volume 300 µL) |
| Indicator filters | 485 nm / 538 nm |
| Reference filters | 485 nm / 620 nm |
Resources
Publications
pH Monitoring in Dairy Starter Cultures
Functional strain redundancy and persistent phage infection in Swiss hard cheese starter cultures
Mikrotiterplatten-Reaktoren mit integrierten pH-Sensoren und Autodisplay in E.coli zur evolutiven Enzymentwicklung
Entwicklung von innovativen Mikrotiterplattenreaktoren zur Bewertung des Abbaus und der Toxizität neuer Wirkstoffe auf Basis von Mikrosomen und Säugerzellen
Scale-down and parallel operation of a riboflavin production process with Bacillus subtilis
FAQs
Are the fluorescence intensities I get from the SensorPlate with my reader sufficient for a good measurement?
How do I import data into excel from a text file containing measurement data?
How do I know that my reader gives precise results with the SensorPlates?
How often do I have to calibrate the SensorPlates with my reader?
Is my microplate reader compatible to the SensorPlates?
The filters of my reader do not exactly match the combination in the SensorPlates' technical data. Nevertheless, can I use them?
The intensities I get with the SensorPlates are very low for my reader. How can I enhance them?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Why are the fluorescence intensities and ratios I get with my reader different from the ones in the SensorPlate manual?