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Fix up to 4 SDRs and Deep Well SensorDishes® on a Shaker
Clamp System for 4 PreSens SDRs
This Clamp System by Enzyscreen fastens up to 4 SensorDish® Readers, Deep Well SensorDishes® and Sandwich covers on a shaker. The system is compatible with most standard shakers and is attached to the shaker tray with screws. The SDRs slide into the system and are held down from above by clamps.
Please contact our service team for more information!
- Measure in shaken cultures with SDR
- Suitable for standard shakers
- Specially modified for the SDR
- Also available for 2 SDRs
Assessing Optimal Growth Conditions for Shaken Yeast Culture
The yeast P. guillermondii was cultivated in deep well Oxo- and HydroDishes to examine growth profiles and determine the minimal filling volumes for sensor read-out at a shaking speed of 200 rpm. As no optical shielding mask (SDR-OSM24) was used in these experiments, recordings show a higher noise-ratio at lower filling volumes. Both oxygen and pH kinetics show clearly when the initial substrate is consumed and the cultures switch to metabolise another substrate. This substrate change correlates with the filling volume, as low volume samples had better oxygen supply and grew faster, so the initial substrate was used up earlier. The optimal working volume for yeast cultivation in the deep well plates is 1.25 - 1.5 mL.
Thomas Grimm, BIOWORX, Berlin, Germany
Evaluating the SDR & OxoDishes® for Strain Development
Oxygen kinetics during a shaken E. coli cultivation were monitored using a Deep Well OxoDish®. Different media compositions were tested and compared to a minimal medium (MM). A higher concentration of yeast extract (YE) led to faster growth. Addition of glycerine as a second substrate prolonged the stationary phase. Ammonium chloride had no influence on the metabolism. After the substrate(s) was consumed, oxygen increased due to oxygen ingress.
Olaf Christensen, Lonza Ltd., Visp, Switzerland
Improving Culture Conditions of Antibody-Producing Yeast
Reliable screening set-ups are the key to rapid identification of genetic modifications or changes of cultivation conditions improving strain performance. Using Deep Well SensorDishes® to improve culture conditions for antibody-producing S. cerevisiae strains, it was found that increasing the buffering capacity of standard synthetic yeast media affects the stability of the secreted antibody. Furthermore, it was detected that the media modification also affected dissolved oxygen levels during batch growth of the strain.
l. Kirjavainen and A. Frey, Aalto University, Finland
|Dimensions||395 x 132 x 160 mm (with carbon clamp)|
for up to 4 SDRs with Deep Well SensorDishes® and covers
SDR SensorDish® Reader Basic Set
The SDR SensorDish® Reader is a small 24-channel reader for…
SDR SensorDish® Reader Extension Set
Up to 9 SDR Extension Sets can be combined with one Basic…
Deep Well OxoDish® OD24-DW
The Deep Well OxoDish® OD24-DW is a disposable polystyrene…
Deep Well HydroDish® HD24-DW
The Deep Well HydroDish® HD24-DW is a disposable polystyrene…
Sandwich Cover for 24-Well Deep Well Plates
This metal cover by Enzyscreen has 24 holes and contains 3…
Optical Shielding Mask SDR-OSM24
The optical shielding mask is designed for use with the SDR…