Watch tutorials, webinars and informative videos about PreSens optical sensor systems.
24 Deep Well Plate with Integrated Oxygen Sensors for Read-Out with the SDR
Deep Well OxoDish® OD24-DW
The Deep Well OxoDish® OD24-DW is a disposable polystyrene multidish and used for shaken applications. Pre-calibrated oxygen sensors are integrated at the flat bottom of each square well and are read out with the SDR SensorDish® Reader. They are delivered beta-irradiated.
- Ready-to-use
- For shaken cultures
- Pre-calibrated
- Manual calibration possible
- For microbes & cell cultures
Applications
Assessing Optimal Growth Conditions for Shaken Yeast Culture
The yeast P. guillermondii was cultivated in deep well Oxo- and HydroDishes to examine growth profiles and determine the minimal filling volumes for sensor read-out at a shaking speed of 200 rpm. As no optical shielding mask (SDR-OSM24) was used in these experiments, recordings show a higher noise-ratio at lower filling volumes. Both oxygen and pH kinetics show clearly when the initial substrate is consumed and the cultures switch to metabolise another substrate. This substrate change correlates with the filling volume, as low volume samples had better oxygen supply and grew faster, so the initial substrate was used up earlier. The optimal working volume for yeast cultivation in the deep well plates is 1.25 - 1.5 mL.
Thomas Grimm, BIOWORX, Berlin, Germany
Evaluating the SDR & OxoDishes® for Strain Development
Oxygen kinetics during a shaken E. coli cultivation were monitored using a Deep Well OxoDish®. Different media compositions were tested and compared to a minimal medium (MM). A higher concentration of yeast extract (YE) led to faster growth. Addition of glycerine as a second substrate prolonged the stationary phase. Ammonium chloride had no influence on the metabolism. After the substrate(s) was consumed, oxygen increased due to oxygen ingress.
Olaf Christensen, Lonza Ltd., Visp, Switzerland
Technical
| Specifications | |
|---|---|
| * in H2O dest. or oxygen-free water | |
| Measurement range | 0 - 50 % O2 |
| Resolution* | ± 0.4 % O2 |
| Precision* | ± 1 % O2 |
| Drift* | < 0.2 % O2 within one week (sampling interval 10 min.) |
| Measurement temperature range | from + 15 °C to + 45 °C |
| Response time* (t90) | at + 25 °C: < 30 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Calibration | Pre-calibrated Disposables are delivered beta-irradiated |
| Maximum filling volume | 10 mL |
Related products
Resources
Publications
Improving Culture Conditions of Antibody-Producing Yeast
Evaluating the SDR SensorDish® Reader for Strain Development
Oxygen and pH Monitoring in Deep Well OxoDishes® and HydroDishes®
HIF activation enhances FCyRIIb expression on mononuclear phagocytes impeding tumor targeting antibody immunotherapy
FAQs
Can I re-use the SensorDishes®?
Can I use the SDR with other formats than the 24-well plates?
Do cells grow on the sensor inside the SensorDishes®?
Do SensorDishes® come sterile?
How can I convert an oxygen value into a different oxygen unit?
How do I import data into excel from a text file containing measurement data?
How does temperature affect the oxygen measurement?
How long can I use a SensorDish®?
How long do I need to equilibrate before starting a SDR measurement?
The SDR software shows "no sensor" instead of a value. What is the reason?
What is the difference between User-Defined Calibration and One-Point Adjustment for the SDR? When do I apply which one?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Which toxicity tests where done with the SensorDish®?
Why are there two different calibration data sets for SensorDishes®?
Why do I need to equilibrate my samples for such long time periods before measurement with the SDR?
Why do I see peaks in the graphs of the SDR software (especially in the oxygen signal) when I open the incubator door / take the plates out of the incubator?