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24 Deep Well Plate with Integrated pH Sensors for Read-out with the SDR
Deep Well HydroDish® HD24-DW
The Deep Well HydroDish® HD24-DW is a disposable polystyrene multidish and used for shaken cultures. Pre-calibrated pH sensors are integrated at the flat bottom of each square well and are read out with the SDR SensorDish® Reader. They are delivered beta-irradiated.
- Ready-to-use
- For shaken cultures
- Pre-calibrated
- Manual calibration possible
- For microbes & cell cultures
Applications
Assessing Optimal Growth Conditions for Shaken Yeast Culture
The yeast P. guillermondii was cultivated in deep well Oxo- and HydroDishes to examine growth profiles and determine the minimal filling volumes for sensor read-out at a shaking speed of 200 rpm. As no optical shielding mask (SDR-OSM24) was used in these experiments, recordings show a higher noise-ratio at lower filling volumes. Both oxygen and pH kinetics show clearly when the initial substrate is consumed and the cultures switch to metabolise another substrate. This substrate change correlates with the filling volume, as low volume samples had better oxygen supply and grew faster, so the initial substrate was used up earlier. The optimal working volume for yeast cultivation in the deep well plates is 1.25 - 1.5 mL.
Thomas Grimm, BIOWORX, Berlin, Germany
Technical
| Specifications | |
|---|---|
| * in physiological solutions, 37 °C | |
| Measurement range | pH 6.0 - 8.5 |
| Resolution* | at pH = 7: ± 0.05 pH |
| Precision* | ± 0.2 pH at pH = 7 (sensor batch calibration) ± 0.1 pH at pH = 7 (sensor spot calibration) |
| Drift* | < 0.1 pH within one week (sampling interval 10 min.) |
| Measurement temperature range | from + 15 to + 45 °C |
| Response time (t90) | at 25 °C: < 120 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 |
| Cross-sensitivity | Reduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere |
| Calibration | Pre-calibrated Disposables are delivered beta-irradiated |
| Maximum filling volume | 10 mL |
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FAQs
Can I re-use the SensorDishes®?
Can I use the SDR with other formats than the 24-well plates?
Do cells grow on the sensor inside the SensorDishes®?
Do SensorDishes® come sterile?
How do I import data into excel from a text file containing measurement data?
How long can I use a SensorDish®?
How long do I need to equilibrate before starting a SDR measurement?
The SDR software shows "no sensor" instead of a value. What is the reason?
What is the difference between User-Defined Calibration and One-Point Adjustment for the SDR? When do I apply which one?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Which toxicity tests where done with the SensorDish®?
Why are there two different calibration data sets for SensorDishes®?
Why do I need to equilibrate my samples for such long time periods before measurement with the SDR?
Why do I see peaks in the graphs of the SDR software (especially in the oxygen signal) when I open the incubator door / take the plates out of the incubator?
Why does the SDR software show pH<5, although I know that my sample is basic?