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Online Oxygen Monitoring in Perfusion Systems
O2 Flow-Through Cell FTC-PSt3
Miniaturized chemical optical oxygen sensors integrated in flow-through cells (FTC-PSt3) allow non-invasive online monitoring of oxygen in perfusion systems. The sensors are fixed to color coded sticks, which can be attached to flow-through cells of different size and shape according to your requirements. A polymer optical fiber connects the sensor inside the flow-through cell to the respective O2 meter (e. g. Fibox 4). The FTC-PST3 cells are made of polycarbonate.
- Precise online monitoring of oxygen
- Different sizes and shapes for various flow rates
- Easy connection to external tubing
Applications
Online Measurement in Perfusion Systems
Beta-irradiated and pre-calibrated pH and DO flow-through sensors can be integrated into perfusion systems. This allows easy control of process parameters in perfusion reactors. Typically Luer connectors are used, though different sizes for larger or smaller flow rates are available.
In-line Oxygen Monitoring in 3D Cell Culture
Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. PreSens flow-through cells with integrated chemical optical sensors can be incorporated in the construct inlet and outlet and allow in-line measurement of oxygen tension.
Application Note by R. Santoro, et al.: Perfusion Culture of Cell Seeded 3D Scaffolds for Tissue Engineering
Technical
| Specifications | Dissolved O2 |
|---|---|
*after two-point calibration as described in the manual | |
| Measurement range | 0 – 45 mg/L 0 – 1400 µmol/L |
| Resolution | ± 0.004 mg/L at 0.091 mg/L ± 0.04 mg/L at 9.1 mg/L |
| Accuracy at + 20 °C* | ± 0.4 % O2 at 20.9 % O2 ± 0.05 % O2 at 0.2 % O2 |
| Drift at 0 % oxygen | < 0.03 % O2 within 30 days (sampling interval of 1 min.) |
| Measurement temperature range | From 0 to + 50 °C |
| Response time (t90)** | < 30 sec. |
| Properties | |
| Compatibility | Aqueous solutions, ethanol, methanol |
| Cross-sensitivity | Organic solvents, such as acetone, toluene, chloroform or methylene chloride Chlorine gas |
| Sterilization procedure*** | Irradiation Ethylene oxide (EtO) |
| Calibration | Sensor sticks are pre-calibrated; Two-point calibration in oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment |
| Storage stability | Up to 60 months provided the sensor material is stored in the dark at room temperature |
| T-cell formats | Luer T-cell (delivered), inner diameter 5 mm, cell volume 0.3 mL; 1/4" x 1/4" (Qosina), cell volume 2.1 mL; 3/8" x 3/8" (Qosina), cell volume 4.6 mL; 1/2" x 1/2" (Qosina), cell volume 8.3 mL |
Related products
Resources
Publications
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Enhanced Oxygen Volumetric Mass Transfer in a Geometrically Constrained Vortex
Increasing ibuprofen degradation in constructed wetlands by bioaugmentation with gravel containing biofilms of an ibuprofen-degrading Sphingobium yanoikuyae
Experimental Sensitivity Analysis of Oxygen Transfer in the Capillary Fringe
FAQs
How can I convert an oxygen value into a different oxygen unit?
How can I prepare the calibration solutions cal0 and cal100 for oxygen sensors?
How do I import data into excel from a text file containing measurement data?
How do I verify whether the oxygen sensor is giving correct readings (performance proof)?
How does an oxygen sensor work?
How does salinity affect the oxygen measurement?
How does temperature affect the oxygen measurement?
The Stern-Volmer-equation
What are the response times for the oxygen sensors based on the 2 mm fiber like non-invasive oxygen sensors and oxygen probes?
What is the maximum flow rate and pressure for your oxygen flow-through cells?
What is the time of delivery?
Which substances can interfere with the optical O2, pH and CO2 measurements?
Manuals
Brochures
Software
PreSens Datamanager version 2.0.0.57 - Windows XP/Vista/7/8
PreSens Oxygen Calculator version 3.1.1 - Windows 7/8/10