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Easy Integration into any Transparent Vessel
Self-adhesive pH Sensor Spot SP-HP5-SA
The new self-adhesive technology simplifies the integration of these sensor spots. They can be mounted in transparent glass or plastic vessels. The pH is measured non-invasively and non-destructively from the outside – through the vessel wall. Optimized for physiological solutions and culture media these sensors are especially suited for bioprocess development or pharmaceutical applications. They are available irradiated or untreated. These sensor spots come pre-calibrated and ready-to-use. One-time autoclaving is possible.
- Simplified integration with new self-adhesive technology
- Compatible with glass & nearly all plastic vessels
- Pre-calibrated & ready-to-use
- Online monitoring without sampling
- Contactless measurements through the vessel wall
Bioprocess Development: pH Monitoring in Shake Flasks
The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used. The combination with non-invasive oxygen sensors in shake flasks provides new insights into metabolic activity and changes in metabolic pathways.
* provided pH sensors are used without further handling in physiological solutions
|Measurement range||5.5 - 8.5 pH|
|Resolution||at pH = 7: ± 0.01 pH|
|Accuracy||at pH = 7: ± 0.05 sensor spot calibration|
|Drift||at pH = 7: < 0.005 pH per day (sampling interval of 1 min.)|
|Measurement temperature range||from + 5 to + 50 °C|
|Response time (t90)**||< 120 sec.|
|Compatibility||Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10|
|Cross-sensitivity||Reduced to ionic strength (salinity); a high cnocentration of small fluorescent molecules in the visible range can interfere|
|Cleaning procedure||pH spots are delivered either beta-irradiated or untreated; one-time autoclaving or Et0 treatment is possible***, a second irradiation or ethylene oxide treatment is not recommended|
|Calibration||pH spots are pre-calibrated|
|Storage stability||18 months provided the sensor is stored in the dark|
|Carrier materials||Adhers to nearly all surfaces like glass, polystyrene, polyethylene, polymethacrylate (acrylic glass), polycarbonate. The sensor does NOT adhere to silicone surfaces!|
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