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Easy Integration into any Transparent Vessel

Self-adhesive pH Sensor Spot SP-HP5-SA

The new self-adhesive technology simplifies the integration of these sensor spots. They can be mounted in transparent glass or plastic vessels. The pH is measured non-invasively and non-destructively from the outside – through the vessel wall. Optimized for physiological solutions and culture media these sensors are especially suited for bioprocess development or pharmaceutical applications. They are available irradiated or untreated. These sensor spots come pre-calibrated and ready-to-use. One-time autoclaving is possible.

  • Simplified integration with new self-adhesive technology
  • Compatible with glass & nearly all plastic vessels
  • Pre-calibrated & ready-to-use
  • Online monitoring without sampling
  • Contactless measurements through the vessel wall

Applications

Bioprocess Development: pH Monitoring in Shake Flasks

The pH is one of the major issues in the cultivation of cells, yeast or bacteria. Shake flask cultures are widely applied in academic and industrial bioprocess development. As adequate methods for real monitoring of pH were not available, cumbersome at-line sampling was used. The combination with non-invasive oxygen sensors in shake flasks provides new insights into metabolic activity and changes in metabolic pathways.


Technical

Specifications*

* provided pH sensors are used without further handling in physiological solutions
** equilibrated sensor kept in well stirred solution at 37 °C
*** recalibration is recommended

Measurement range5.5 - 8.5 pH
Resolutionat pH = 7: ± 0.01 pH
Accuracyat pH = 7: ± 0.05 sensor spot calibration
Driftat pH = 7: < 0.005 pH per day (sampling interval of 1 min.)
Measurement temperature rangefrom + 5 to + 50 °C
Response time (t90)**< 120 sec.
Properties*
CompatibilityAqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Cross-sensitivityReduced to ionic strength (salinity); a high cnocentration of small fluorescent molecules in the visible range can interfere
Cleaning procedurepH spots are delivered either beta-irradiated or untreated; one-time autoclaving or Et0 treatment is possible***, a second irradiation or ethylene oxide treatment is not recommended
CalibrationpH spots are pre-calibrated
Storage stability18 months provided the sensor is stored in the dark
Carrier materialsAdhers to nearly all surfaces like glass, polystyrene, polyethylene, polymethacrylate (acrylic glass), polycarbonate. The sensor does NOT adhere to silicone surfaces!

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