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Online Oxygen Monitoring in 20 mL Vials

SensorVial SV-PSt3-20mL-NST

A sensor stripe is integrated in this 20 mL glass vial. The sensor is read out with an optical fiber, which is held in place by the Vial Adapter. The Vial Adapter can be positioned in different heights. The SensorVial SV-PSt3-20mL-NST is autoclavable, and the ideal tool for respiration measurements. Since the sensor stripes are optically isolated with a black layer they are also very well suited for photosynthesis measurements. For stirred applications the SensorVial is also available with a sensor stripe that does not reach all the way to the bottom. Oxygen can be measured simultaneously in liquid and headspace.

  • Non-invasive online oxygen measurements
  • No consumption of oxygen
  • Signal independent of flow velocity
  • Oxygen measurements in liquids and gas phase
  • Autoclavable

Applications

Respiration & Photosynthesis: Oxygen Monitoring in Glass Vials

Determination of respiratory activity is often performed for water organisms such as invertebrates, larval stages or eggs, but also for bacteria, cell cultures, yeasts or fungi. For algae measurement of photosynthetic activity is of great interest. Using our 20 mL SensorVial with an integrated sensor stripe oxygen can be measured simultaneously in the liquid sample and in the headspace. Autoclavable SensorVials for stirred and non-stirred applications are available.


Technical

SpecificationsGaseous & Dissolved O2Dissolved O2
*after two-point calibration as described in the manual
Measurement range

0 – 100 % O2

0 – 1000 hPa

0 – 45 mg/L

0 – 1400 µmol/L
Limit of detection0.03 % oxygen15 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.1 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.4 µmol/L at 283.1 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.03 % O2 within 30 days (sampling interval of 1 min. / at 0% oxygen)
Measurement temperature rangefrom 0 to + 50 °C
Response time (t90)< 6 sec.< 40 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure Steam sterilization
Ethylene oxide (EtO)
Gamma irradiation
Cleaning procedure Cleaning in place (CIP, 2 % NaOH, + 80 °C, + 176 °F)
3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite)and air-saturated environment
Storage stability24 months provided the sensor material is stored in the dark

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