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Online Oxygen Monitoring in Perfusion Systems

Single-Use O2 Flow-Through Cell FTC-SU-PSt3-S

Miniaturized chemical optical oxygen sensors integrated in single-use flow-through cells (FTC-SU-PSt3) allow non-invasive online monitoring of oxygen in perfusion systems. The sensors are fixed to color coded sticks, which can be attached to flow-through cells of different size and shape, according to your requirements. A polymer optical fiber connects the sensor inside the flow-through cell to the respective O2 meter (e. g. Fibox 4). The FTC-SU-PST3-S single-use cells are made of polycarbonate and are delivered beta-irradiated.

  • Single-use flow-through cells
  • Precise online monitoring of oxygen
  • Different sizes and shapes for various flow rates
  • Easy connection to external tubing
  • Beta-irradiated

Applications

In-line Oxygen Monitoring in 3D Cell Culture

Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. PreSens flow-through cells with integrated chemical optical sensors can be incorporated in the construct inlet and outlet and allow in-line measurement of oxygen tension.

Application Note by R. Santoro, et al.: Perfusion Culture of Cell Seeded 3D Scaffolds for Tissue Engineering


Technical

SpecificationsDissolved O2

*after two-point calibration as described in the manual
**equilibrated FTC with physiological solution and sufficient flow rate (min. 15 mL/min) at 37 °C
***recalibration may be required

Measurement range

0 –  45 mg/L

0 – 1400 µmol/L
Resolution± 0.004 mg/L at 0.091 mg/L
± 0.04 mg/L at 9.1 mg/L
Accuracy at + 20 °C*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift at 0 % oxygen< 0.03 % O2 within 30 days (sampling interval of 1 min.)
Measurement temperature rangeFrom 0 to + 50 °C
Response time (t90)**< 30 sec.
Properties
CompatibilityAqueous solutions, ethanol, methanol
Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure***

Irradiation

Ethylene oxide (EtO)
Calibration

Sensor sticks are pre-calibrated;

Two-point calibration in oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stabilityUp to 60 months provided the sensor material is stored in the dark at room temperature
T-cell formats Luer T-cell (delivered), inner diameter 5 mm, cell volume 0.3 mL;
1/4" x 1/4" (Qosina), cell volume 2.1 mL;
3/8" x 3/8" (Qosina), cell volume 4.6 mL;
1/2" x 1/2" (Qosina), cell volume 8.3 mL

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