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Fast Oxygen Monitoring in Perfusion Systems

Flow-Through Cell Housed Oxygen Microsensor FTCH-PSt1

The oxygen microsensor housed in a flow-through cell is optimized for a fast response time (t90< 1 sec in gas, < 5 sec. in liquid). The microsensor with a tip size from < 50 µm to 140 µm is integrated in a sleeve made of Teflon® tightened with a silicone rubber, which is mounted to a T-connector. The liquid volume inside the FTCH is about 310 µL. External tubing with an inner diameter of 1.6, 2.3, and 3.2 mm can be connected via Luer-Lock adpaters. Liquids (e. g. water, blood, etc.) can be pumped through the cell. The FTCH-PSt1 has a measurement range of 0 – 50 % oxygen, with a detection limit of 0.05 % oxygen.

  • Perfusion monitoring in smallest volumes
  • High temporal resolution (< 5 sec.)
  • No consumption of oxygen
  • Measures in liquids as well as in the gas phase

Technical

SpecificationsGaseous & Dissolved O2Dissolved O2

*after two-point calibration as described in the manual
**TS: tapered sensor tip with a diameter < 50 μm and no optical isolation
***TF: flat-broken sensor tip with a diameter of 140 μm

Measurement range

0 – 50 % O2

0 – 500 hPa

0 – 22.5 mg/L

0 – 700 µmol/L
Limit of detection0.05 % oxygen20 ppb
Resolution

± 0.01 % O2 at 0.21 % O2
± 0.09 % O2 at 20.9 % O2
±0.1 hPa at 2 hPa

± 1 hPa at 207 hPa

± 0.005 mg/L at 0.09 mg/L
± 0.04 mg/L at 9.06 mg/L
± 0.14 µmol/L at 2.83 µmol/L

± 1.3 µmol/L at 283 µmol/L
Accuracy*± 0.4 % O2 at 20.9 % O2
± 0.05 % O2 at 0.2 % O2
Drift< 0.1 % O2 within 30 days (sampling interval of 1 min.)
Measurement temperature rangefrom 0 to + 50 °C
Response time TS** (t90)< 1 sec. (gas)< 2 sec. (liquid)
Response time TF*** (t90)< 15 sec. (gas)< 30 sec. (liquid)
Properties
CompatibilityAqueous solutions, ethanol, methanol
No cross-sensitivity

pH 1 – 14
CO2, H2S, SO2
Ionic species

Cross-sensitivityOrganic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure

Ethylene oxide (EtO)

Cleaning procedure3 % H2O2
Ethanol
Soap solution
CalibrationTwo-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability60 months provided the sensor material is stored in the dark at room temperature

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